Neuroprotective effect of epigallocatechin-3-gallate on hemisection-induced spinal cord injury in rats  

作　　者：Fengjun Deng Rubing Li Yingbao Yang Dan Zhou Qian Wang Jiangping Xu 

机构地区：[1]Department of Pharmaceutical Sciences, Hunan Yiyang Medical College, Yiyang 413000, Hunan Province, China [2]Pharmaceutical Preparation Section, General Hospital of Guangzhou Military Command of PLA, Guangzhou 510010, Guangdong Province, China [3]Department of Pharmacology, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China [4]Pingxiang Healthy School, Pingxiang 337000, Jiangxi Province, China

出　　处：《Neural Regeneration Research》2011年第6期405-411,共7页中国神经再生研究（英文版）

摘　　要：Epigallocatechin-3-gallate （EGCG）, a naturally occurring compound in green tea, has been widely used as an antioxidant agent. In the present study, model rats with acute spinal cord injury were intraperitoneally injected with 25, 50, and 100 mg/kg EGCG, and spinal cord ultrastructure, oxidative stress reaction, inflammatory factors, and apoptosis-associated gene expression were observed. Results showed that EGCG attenuated neuronal and axonal injury 24 hours post injury. It also decreased serum intedeukin-113, tumor necrosis factor-a, and intercellular adhesion molecule-1 release, and decreased apoptosis-associated gene expression. Furthermore, it increased the level of the superoxide anion （O2-）, superoxide dismutase, and B-cell lymphoma/leukemia-2, and reduced malondialdehyde levels. Furthermore, it reduced the expression of the pro-apoptotic protein Bax. Noticeably, EGCG at the 100 mg/kg dosage exhibited similar effects as methylprednisolone sodium succinate, which has been frequently used for clinical acute spinal cord injury. The results demonstrated that EGCG can significantly inhibit inflammation, suppress oxidation, and reduce apoptosis in acute spinal cord injury.Epigallocatechin-3-gallate （EGCG）, a naturally occurring compound in green tea, has been widely used as an antioxidant agent. In the present study, model rats with acute spinal cord injury were intraperitoneally injected with 25, 50, and 100 mg/kg EGCG, and spinal cord ultrastructure, oxidative stress reaction, inflammatory factors, and apoptosis-associated gene expression were observed. Results showed that EGCG attenuated neuronal and axonal injury 24 hours post injury. It also decreased serum intedeukin-113, tumor necrosis factor-a, and intercellular adhesion molecule-1 release, and decreased apoptosis-associated gene expression. Furthermore, it increased the level of the superoxide anion （O2-）, superoxide dismutase, and B-cell lymphoma/leukemia-2, and reduced malondialdehyde levels. Furthermore, it reduced the expression of the pro-apoptotic protein Bax. Noticeably, EGCG at the 100 mg/kg dosage exhibited similar effects as methylprednisolone sodium succinate, which has been frequently used for clinical acute spinal cord injury. The results demonstrated that EGCG can significantly inhibit inflammation, suppress oxidation, and reduce apoptosis in acute spinal cord injury.

关 键 词：epigallocatechin-3-gallate spinal cord injury neuroprotective effect oxidative stress INFLAMMATION apoptosis 

分 类 号：Q257[生物学—细胞生物学] S857.18[农业科学—临床兽医学]