RT-PCR扩增Sj26、Sj32和Sj14-3-3抗原编码基因诊断急性日本血吸虫病的初步研究  

Diagnosis of acute Schistosomiasis japonica by amplifying Sj26,Sj32 and Sj14-3-3 encoding gene using RT-PCR

作　　者：王敏[1] 李文桂[1] 蔡世飞[1] 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,重庆400016

出　　处：《中国人兽共患病学报》2011年第3期229-232,共4页Chinese Journal of Zoonoses

基　　金：重庆市科委地方病重大项目(2008AB5055;2008AA5008和2008AB5054)

摘　　要：目的探讨RT-PCR扩增Sj26、Sj32和Sj14-3-3抗原编码基因用于急性日本血吸虫病患者的诊断价值。方法从急性日本血吸虫病患者血清中提取总RNA,利用RT-PCR方法扩增Sj26、Sj32和Sj14-3-3抗原编码基因,用1.2%琼脂糖凝胶电泳进行鉴定,同时以华支睾吸虫病患者血清、卫氏并殖吸虫病患者血清和正常人血清总RNA作为对照。结果 50份急性日本血吸虫病血清均通过RT-PCR扩增出了400bp的Sj14-3-3抗原编码基因片段,但未扩增出676bp的Sj26和1 270bp的Sj32抗原编码基因片段;对照组呈阴性反应。RT-PCR扩增Sj14-3-3抗原编码基因片段敏感性和特异性均为100%,与华支睾吸虫病和卫氏并殖吸虫病患者血清未见交叉反应。结论 RT-PCR扩增Sj14-3-3抗原编码基因可用于急性日本血吸虫病的基因诊断。In order to investigate the value of amplifying Sj26,Sj32 and Sj14-3-3 encoding gene by RT-PCR for diagnosis patients with acute schistosomiasis japonica,the total RNA was extracted from the sera of patients with acute schistosomiasis japonica.The antigen encoding gene of Sj26,Sj32 and Sj14-3-3 were amplified by RT-PCR from the total RNA and identified by 1.2% AgaroseⅡ.At the same time,the total RNA were obtained from the sera of patients with paragonimiasis westermani,clonorchiasis sinensis and the normal subjects as the control groups.A 400bp Sj14-3-3 encoding gene was obtained by RT-PCR from the 50 schistosomiasis japonica patients,but the encoding gene of Sj26 and Sj32 weren＇t obtained,and the control groups were negative.The sensitivity and specialty were both 100% by RT-PCR with Sj14-3-3.There was no cross reaction between clonorchiasis sinensis and paragonimiasis westermani.It＇s indicated that the amplification of Sj14-3-3 encoding gene might be used for the gene diagnosis of patients with acute Schistosomiasis japonica.

关 键 词：日本血吸虫病 RT-PCR 基因诊断 

分 类 号：R532.2[医药卫生—内科学]