苯酚-硫酸法测定藏木香中多糖含量  

Determination of the Content of Polysaccharide in Inula Helenium by Phenol-Sulfuric Acid Method

作　　者：哈文秀[1] 利毛才让[1] 热增才旦[2] 

机构地区：[1]青海师范大学民师院化学系,青海西宁810008 [2]青海大学医学院中藏药研究中心,青海西宁810001

出　　处：《青海师范大学学报（自然科学版）》2011年第1期35-37,共3页Journal of Qinghai Normal University(Natural Science Edition)

摘　　要：采用传统的水煮醇沉法从藏木香中提取可溶性多糖,通过Sevag法脱蛋白后,以葡萄糖为对照品,使用苯酚-硫酸法测定多糖含量.结果表明,在波长486nm处测定吸光度,10-100μg/mL范围内吸光度与被测含量之间具有良好的线性关系,藏木香中多糖的含量为64.37%.The soluble polysaccharides in Inula helenium were extracted by the traditional method of boiling with water and depositing in alcohols.After removing proteins,the content of polysaccharides was determined by phenol-sulfuric acid method with glucose as standard sample.The result showed that there were favorable linearity relationship between the absorption and content determined in the range of 10-100μg/mL at the wavelength of 486nm.The content of polysaccharides was about 64.37%.

关 键 词：藏木香 多糖 苯酚-硫酸法 

分 类 号：O656.22[理学—分析化学]