粟米草三帖皂苷对PDGF诱导的血管平滑肌细胞增殖的影响  

Mollugo Pentaphylla Triterpenes Saponins' effects on vascular smooth muscle cell proliferation induced by platelet-derived growth factor

作　　者：干丽君[1] 龚帧[2] 熊建军[2] 李雪芹[2] 吴周环[2] 林玲[2] 李卫东[2] 

机构地区：[1]九江学院护理学院,江西九江332000 [2]九江学院基础医学院,江西九江332000

出　　处：《中国现代医学杂志》2011年第8期947-950,共4页China Journal of Modern Medicine

基　　金：江西省科技厅自然科学基金资助项目(No:2008ZGY0096);江西省卫生厅科技计划项目(No:20073214)

摘　　要：目的研究粟米草三帖皂苷(MPTS)在体外对血小板源生长因子(PDGF)诱导大鼠血管平滑肌细胞(VSMCs)增殖作用的影响及其机制。方法原代培养大鼠血管平滑肌细胞,PDGF或PDGF与不同浓度MPTS(1￣27μmol/L)共同作用后,以MTT法检测细胞活力;western-blot检测细胞pERK1/2表达变化。结果成功培养血管平滑肌细胞;10 ng/mL的PDGF促进VSMCs的增殖,而不同浓度MPTS作用后均可抑制PDGF诱导的VSMCs增殖(P<0.05);并且,MPTS对PDGF诱导的pERK1/2活化有抑制作用(P<0.05)。结论 MPTS对PDGF诱导的大鼠血管平滑肌细胞的增殖有抑制作用,其部分机制可能与下调pERK1/2活性有关。【Objective】 To investigate the effects of Mollugo Pentaphylla Triterpenes Saponins（MPTS） on proliferation of vascular smooth muscle cells（VSMCs） induced by PDGF in vitro,and to explore the underlying mechanism.【Methods】 The primary cultured rat VSMCs were exposed to PDGF or PDGF combining various concentrations of MPTS（1～27 μmol/L）,and the proliferative activity of cells were determined by MTT assay.The effects of MPTS on expression of pERK1/2 were detected by western blot.【Results】 The primary VSMCs were successfully cultured.In the concentration of 10 ng/mL,exogenous PDGF promoted the proliferation of VSMCs.However,various concentrations of MPTS inhibited proliferative activity of PDGF on VSMCs（P 0.05）.Furthermore,MPTS decreased the activation of pERK1/2 induced by PDGF（P 0.05）.【Conclusion】 MPTS can inhibit the PDGF-induced growth of VSMCs,decrease of pERK1/2 activation might be one of its mechanisms.

关 键 词：粟米草三帖皂苷 血小板源生长因子 ERK1/2 细胞增殖 

分 类 号：R-332[医药卫生]