黄鳝Ⅲ型Hepcidin基因cDNA克隆及序列分析  

Cloning and Sequence Analysis of Type Ⅲ Hepcidin cDNA from Rice Field Eel(Monopterus albus)

作　　者：李伟[1] 李长朋[1] 

机构地区：[1]长江大学生命科学学院,湖北荆州434025

出　　处：《湖北农业科学》2011年第6期1239-1241,1246,共4页Hubei Agricultural Sciences

基　　金：湖北省教育厅优秀中青年基金项目(Q200712005);长江大学博士创新基金(2006A00433)

摘　　要：以黄鳝为材料,提取肝脏总RNA,经RT-PCR扩增出Hepcidin基因cDNA的开放阅读框(ORF)及3′端非编码区序列(GenBank序列号FJ436807),序列分析表明,黄鳝Hepcidin含有一个273 bp的ORF,编码90个氨基酸残基,有信号肽(24个残基)、前体肽(40个残基)和成熟肽(26个残基)。在前肽部分具有前肽转化酶典型的RX(K/R)R基元。同源性比对表明,黄鳝Hepcidin与其他鱼类的同源性在40%以上,是Hepcidin抗菌肽家族的新成员。Hepcidin＇s open reading frame（ORF） and 3＇ end UTR were amplified from the total RNA of liver of rice field eel by RT-PCR and RACE with specific primers based upon the homology DNA sequence FJ436807 in GenBank.The length of the cloned cDNA was 580 bp with a 273 bp ORF encoding 90 amino acids.The prepropeptide consisted of a signal peptide（24 amino acids）,a prodomain（40 amino acids） and a mature peptide（26 amino acids）.The propeptide contained the typical.Relement of propeptide invertase,mature peptide part contained 6 conserved cysteine residues that could form 3 intrachain disulfide bond.According to the alignment with other Hepcidins,the deduced amino acid sequence of rice field eel Hepcidin share high identity of 40% with those of other species,suggesting it was a new member of Hepcidin family.

关 键 词：黄鳝 抗菌肽 HEPCIDIN CDNA克隆 序列分析 

分 类 号：Q959.482[生物学—动物学] Q785