酶法水解麦冬多糖及其产物的分离鉴定  

Enzymatic Hydrolysis of Ophiopogon japonicus Polysaccharide and Isolation,Identification of the Products

作　　者：吕宁[1] 金凤燮[1] 鱼红闪[1] 

机构地区：[1]大连工业大学生物工程学院

出　　处：《食品与发酵工业》2011年第3期9-12,共4页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(30371744;30470055;20476017);辽宁省高等学校优秀人才支持计划项目(RC204205);辽宁省科学技术基金项目(20042132)

摘　　要：采用生物酶法水解纯化后的麦冬多糖,并对其产物的成分进行分析。利用葡聚糖凝胶Sephadex G-100柱对麦冬粗多糖进行分离纯化,得到相对分子质量为48 347的纯麦冬多糖。使用Absidiasp.O8 s菌产酶水解纯麦冬多糖,产物经Sephadex G-100和Sephadex G-50柱分离,得到OP-1、OP-2、OP-3三种组分,相对分子质量分别为32 452、9 231和1 354。通过高效液相色谱分析纯麦冬多糖及其水解产物的单糖组成,结果表明,纯麦冬多糖的单糖组成为葡萄糖和果糖,其水解产物OP-1、OP-2、OP-3三种组分的单糖组成分别为果糖、果糖、葡糖糖和果糖。The Ophiopogon japonicus polysaccharide was hydrolyzed by bio-enzyme and the products were analyzed. The relative molecular weight of the Ophiopogon japonicus polysaccharide was 48347 , which was purified by Sephadex G-100. The Ophiopogon japonicus polysaccharide was hydrolyzed by enzyme of Absidia sp. O8s, and the products were then isolated using Sephadex G-100 and Sephadex G-50 to generate three components： OP-1,OP-2 and OP-3. Their relative molecular weights were 32452,9231,1354, respectively. From HPLC, the purified Ophiopogon japonicus polysaccharide was mainly composed of glucose and fructose, then, OP-1,OP-2,OP-3 respectively consisted of fructose, fructose, glucose and fructose.

关 键 词：麦冬多糖 酶法水解 相对分子质量 高效液相色谱 

分 类 号：TQ461[化学工程—制药化工]