贴梗海棠花青苷组成及其与花色的关系  

Identification of Anthocyanins Involving in Petal Coloration in Chaenomeles speciosa Cultivars

作　　者：张洁[1,2] 王亮生[2] 高锦明[1] 李圣波 徐彦军[4] 李崇晖[2,5] 杨瑞珍[2,5] 

机构地区：[1]西北农林科技大学理学院,陕西杨凌712100 [2]中国科学院植物研究所北京植物园,北京100093 [3]山东亚特生态技术有限公司,山东临沂276017 [4]中国农业大学理学院,北京100193 [5]中国科学院研究生院,北京100049

出　　处：《园艺学报》2011年第3期527-534,共8页Acta Horticulturae Sinica

摘　　要：以贴梗海棠[Chaenomeles speciosa(Sweet)Nakai]不同花色的24份种质为材料,调查了其花色在CIE L*a*b*表色系的分布状况,利用高效液相色谱-光电二极管阵列检测(HPLC-DAD)和高效液相色谱-电喷雾离子化-多级质谱联用技术(HPLC-ESI-MSn)定性定量分析其花青苷组分,运用多元线性回归方法分析了花色与花青苷组成之间的关系。结果表明,贴梗海棠花瓣中共含有6种花青苷,分别是矢车菊素-3-O-半乳糖苷(Cy3Ga)、矢车菊素-3-O-葡萄糖苷、天竺葵素-3-O-半乳糖苷、天竺葵素-3-O-(半乳糖葡萄糖苷)[Pg3(Ga-G)]、矢车菊素-3-O-(半乳糖葡萄糖苷)以及矢车菊素-3-O-琥珀酸-阿拉伯糖苷(Cy3SucAra)。Cy3SucAra为首次发现。其中,Cy3Ga、Pg3(Ga-G)和Cy3SucAra是决定贴梗海棠花色的主要色素,这3种色素含量的增加导致花色显著变红。基于花青苷的组成信息,探讨了贴梗海棠的花色改良和蓝色花创制的策略。Coloration and anthocyanins of petals collected from Chaenomeles speciosa（Sweet）Nakai of 24 accessions were determined. Color was measured by CIE L＊a＊b＊ scale and anthocyanins composition of C. speciosa was determined using high-performance liquid chromatography coupled with photodiode array detection（HPLC–DAD）and HPLC electrospray ionization mass spectrometry（HPLC–ESI–MSn）. Cyanidin-3-O-galactoside（Cy3Ga）,cyanidin-3-O-glucoside,pelargonidin-3-O-galactoside,pelargonidin-3-O-（galactose-glucose）[Pg3（Ga-G）],cyanidin-3-O-（galactose-glucose）and cyanidin-3-O- succinylarabinoside（Cy3SucAra）were detected. Cy3SucAra was identified in C. speciosa for the first time. We explored the relationship between petal colors and anthocyanin contents by multiple linear regression analyses. The results indicated that Cy3Ga,Pg3（Ga-G）and Cy3SucAra were important pigments,high contents of which significantly enhanced the red color of petals. Based on anthocyanins constitutions,flower color modification and blue color breeding for C. speciosa were discussed.

关 键 词：贴梗海棠 花青苷 高效液相色谱 质谱 花色形成机制 

分 类 号：S685.99[农业科学—观赏园艺]