DNA修复基因Rad51和ERCC1蛋白在人肺鳞癌中的表达意义  

Significance of Rad51 and ERCC1 Protein Expression in the Human Lung Squmous Cell Cancers.

作　　者：许俊[1] 高俊[2] 陈洪雷[2] 

机构地区：[1]武汉市医疗救治中心外科,430023 [2]武汉大学基础医学院病理学教研室

出　　处：《医学研究杂志》2011年第3期31-34,共4页Journal of Medical Research

基　　金：国家自然科学基金资助项目(30900652)

摘　　要：目的探讨DNA修复基因Rad51和ERCC1蛋白表达与肺鳞癌临床病理特征的关系。方法采用二步法(非生物素)检测系统结合组织芯片技术检测60例肺鳞癌和15例癌旁肺组织中Rad51和ERCC1的蛋白表达。结果在肺鳞癌组织中Rad51和ERCC1蛋白阳性表达率分别为33.33%(20/60)、41.67%(25/60),均显著高于癌旁肺组织(P<0.05)。但Rad51和ERCC1蛋白阳性表达率与肺鳞癌的临床病理参数均无关(P>0.05)。60例肺鳞癌组织中,Rad51和ERCC1蛋白共同阳性表达14例,共同阴性表达29例,二者呈显著正相关(r=0.406,P=0.001)。结论肺鳞癌中存在Rad51和ERCC1蛋白表达,二者协同作用可能与肺鳞癌的发生发展有关。Objective This study was designed to investigate the expression of DNA repair gene Rad51 protein and its relationship with clinicopathologic features of lung squamous cell carcinoma. Methods Rad51 protein was detected by a two - step （ non - biotin） detection system combined with tissue microarray technique in 60 cases of lung squamous cell carcinomas and 15 cases of para - cancerous lung tissues. Results In lung squamous cell carcinoma tissues, the positive rates of Rad51 and ERCC1 protein were 33.33% （20/60） and 41.67% （25/60）, respectively,which were significantly higher than those of para - cancerous lung tissues （P 〈 0.05）. However, no relation was observed between Rad51, ERCC1 protein and the clinicopathologic features of lung squamous cell carcinoma （ P 〉 0.05 ）. But positive correlation was found between Rad51 and ERCC1 protein （r = 0. 406, P = 0. 001 ）. Conclusion Expression of Rad51 and ERCC1 protein could be detected in the lung squamous cell carcinoma tissues. The synergistic action of Rad51 and ERCC1 protein may correlate with the development and progress of lung squamous cell carcinoma.

关 键 词：肺肿瘤 RAD51 ERCC1 组织芯片 

分 类 号：R734.2[医药卫生—肿瘤]