干细胞相关因子在大肠癌组织及SW620细胞株的表达  

Expression of stem cell-associated genes in colorectal cancer and SW620 cell line

作　　者：李秋娴[1] 黄颖峰[2] 魏亚明[3] 王晓华[1] 罗宏图[3] 杨邵宇[3] 许艳丽[3] 黄于艺[1] 叶亨[1] 

机构地区：[1]广州医学院生化教研室,广东省广州市510180 [2]广州市第一人民医院普外科,广东省广州市510180 [3]广州市临床医学研究所广州市第一人民医院,广东省广州市510180

出　　处：《中国组织工程研究与临床康复》2011年第6期1015-1019,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省医学科研基金(A2008517)课题名称:消化道肿瘤患者外周血中癌细胞;癌干细胞检测分离与临床应用;广州市科技计划项目(2008J1-C171)课题名称:大肠癌实体与外周血癌干细胞分离及其Bmi-1基因表达调控~~

摘　　要：背景:肿瘤细胞有多种干细胞标记的表达,深入研究其表达规律有助于揭示肿瘤发病机制、完善肿瘤干细胞理论。目的:检测大肠癌实体组织中干细胞相关分子标记CD133、CD166、Oct4、Sox2、C-myc、Klf4、Bmi-1与癌旁组织的表达差异,以及CD133免疫磁珠分选阳性与阴性SW620细胞中上述基因的表达差异。方法:选临床病理诊断清楚的29例大肠癌组织标本提取RNA,RT-PCR检测CD133、CD166、Oct4、Sox2、C-myc、Klf4、Bmi-1在大肠癌组织与癌旁对照组织中的表达。CD133免疫磁珠分选SW620,提取CD133细胞与CD133阴性细胞RNA,RT-PCR检测分选后上述基因的表达差异。结果与结论:29例标本均诊断为低、中分化腺癌或黏液腺癌,干细胞相关分子标记癌组织与癌旁组织表达灰度相对值癌组织表达均高于癌旁组织(P<0.05)。RT-PCR检测分选后CD133+细胞CD133,CD166,Oct4,Sox2,C-myc,Klf4,Bmi-1表达,结果均高于CD133-细胞。提示,CD133、CD166、Oct4、Sox2、C-myc、Klf4、Bmi-1相关干细胞标记可作为大肠癌肿瘤干细胞标记并有望用于诊断检测。BACKGROUND：Tumor cells always show some stem cell markers.The expression of these markers will help us to understand the mechanism of tumorgenesis and to perfect the theory of tumor stem cells.OBJECTIVE：To investigate the expression of stem cell-related molecules markers CD133,CD166,Oct4,Sox2,C-myc,Klf4 and Bmi-1 in colorectal cancer tissue,the difference compared with the expression of adjacent tissues,as well as the difference in expression of above-described genes in sorted CD133＋ SW620 cell line.METHODS：RNA was extracted from cancer tissues of 29 colon cancer tissue specimens.The expression of CD133,CD166,Oct4,Sox2,C-myc,Klf4 and Bmi-1 was detected by RT-PCR in colon cancer tissues and adjacent tissues.CD133＋ SW620 cells were sorted,and RNA was extracted from CD133＋ cells and CD133-cells.The different expressions of above genes were detected using RT-PCR.RESULTS AND CONCLUSION：All samples were proved to be poorly/moderately differentiated adenocarcinoma or myxoadenocarcinoma in 29 cases.The relative quantity expressions of the stem cell-related molecules markers in the cancer tissue were higher than adjacent tissues（P 0.05）.RT-PCR indicated that stem cell associated genes expressions（CD133,CD166,Oct4,Sox2,C-myc,Klf4,Bmi-1） in CD133＋ sorted cells were higher than that in CD133-ones.These suggested that CD133,CD166,Oct4,Sox2,C-myc,Klf4 and Bmi-1 were used in colorectal cancer diagnosis.

关 键 词：大肠癌 肿瘤干细胞 CD133 CD166 OCT4 SOX2 KLF4 C-myc BMI-1 

分 类 号：R394.2[医药卫生—医学遗传学]