骨髓间充质干细胞移植膝类风湿关节炎兔:关节滑膜体积和软骨厚度的MRI评价  

Bone marrow mesenchymal stem cells transplantation in treatment of rheumatoid arthritis in rabbit knee joints:Magnetic resonance imaging of cartilage thickness and synovium volume of the knee joints

作　　者：崔延安[1] 刘钊[1] 刘红霞[1] 黄海青[1] 张全彬[2] 

机构地区：[1]江苏省中医院MR室,江苏省南京市210029 [2]南京医科大学附属南京第一医院骨科中心,江苏省南京市210006

出　　处：《中国组织工程研究与临床康复》2011年第6期1024-1029,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：江苏省自然科学基金面上项目(BK2009460);MRI对兔膝关节软骨损伤及BMSCs治疗效果评价的实验研究~~

摘　　要：背景:骨髓间充质干细胞具有显著的免疫调节功能和多向分化潜能,可抑制类风湿关节炎的炎症反应,促进软骨损伤的修复。目的:用骨髓间充质干细胞治疗兔早期类风湿关节炎,运用MRI观察软骨厚度及滑膜体积的改变,评价治疗效果。方法:选取42只新西兰大白兔,用卵蛋白制造类风湿关节炎模型,造模第4周(治疗前)取6只兔行MRI和病理检查以对照,余36只兔随机分模型组和骨髓间充质干细胞治疗组,两组于治疗后1,2,3个月分别选取6只兔行MRI和病理检查。对病变膝关节的滑膜体积和软骨厚度进行MRI测量及病理学评分。结果与结论:治疗1,2,3个月,类风湿关节炎组滑膜进行性增厚,软骨厚度变薄,病理学评分增加;骨髓间充质干细胞治疗组滑膜增厚程度减轻,关节软骨厚度恢复,病理学评分减低。MRI测量数据和病理学评分有相关性。提示MRI可用于评价骨髓间充质干细胞对早期类风湿关节炎的疗效。BACKGROUND：Bone marrow mesenchymal stem cells（BMSCs） have immunoregulatory functions and the potential multiple differentiations.It can inhibit inflammatory reaction of rheumatoid arthritis and promote the repair of cartilage damage.OBJECTIVE：To observe the thickness of cartilage and the volume of synovium in MRI to analyze the effects of BMSCs transplantation on treatment of early rheumatoid arthritis in rabbits.METHODS：Forty-two New Zealand white rabbits were induced by injecting ovalbumin into right knee joints.MR and pathology examination of knee joints in six rabbits were done at 4 weeks after injection（before treatment）.The remaining thirty-six rabbits were randomly and equally divided into model group and BMSCs treatment group.MR and pathology examination of knee joints of 6 rabbits from either group were done at 1,2,3 months after injection.The thickness of cartilage and the volume of synovium of the knee joints were measured by MRI,and pathohistological changes were observed as well.RESULTS AND CONCLUSION：Model group after 1,2,3 months treatment showed synovium thickening and cartilage thinning,and higher pathological scores,and exhibited synovium thinning and cartilage thickening,and lower pathological scores in BMSCs treatment group.The correlation between MRI and pathology was remarkable.These indicated that MRI can show the effect of BMSCs on the early rheumatoid arthritis.

关 键 词：类风湿关节炎 磁共振成像 兔 骨髓间充质干细胞 软骨 滑膜 

分 类 号：R394.2[医药卫生—医学遗传学]