嗅鞘细胞移植联合督脉电针治疗大鼠前脊髓综合征  

Olfactory ensheathing cells transplantation combined with Du meridian electro-acupuncture for treatment of anterior spinal cord injury in rats

作　　者：张淑莲[1] 闫金玉[1] 李剑锋[1] 

机构地区：[1]内蒙古医学院第二附属医院康复科,内蒙古自治区呼和浩特市010030

出　　处：《中国组织工程研究与临床康复》2011年第6期1040-1043,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30860300)~~

摘　　要：背景:电针在脊髓损伤的康复过程中有其一定的临床作用,同时以嗅鞘细胞为代表的细胞治疗在部分患者的康复过程中亦起到了一定的作用,两者是否具有协同应用尚不清楚。目的:观察督脉电针联合嗅鞘细胞移植对前脊髓综合征大鼠神经营养因子3水平及P75NTR表达的影响。方法:将40只成年大鼠随机分为对照组、督脉电针组、嗅鞘细胞移植组、督脉电针+嗅鞘细胞移植组,经嗅鞘细胞移植和督脉电针治疗2周后ELISA法检测脊髓组织神经营养因子3水平,免疫组化检测P75NTR的表达。结果与结论:督脉电针+嗅鞘细胞移植组神经营养因子3水平较对照组及其他实验组高(P<0.05);嗅鞘细胞移植组和督脉电针+嗅鞘细胞移植组的脊髓损伤部位以及相邻的组织内均有P75NTR表达,且督脉电针+嗅鞘细胞移植组阳性表达明显较嗅鞘细胞移植组多。实验证实督脉电针联合嗅鞘细胞移植能够明显增高大鼠前脊髓综合征邻近组织的神经营养因子3水平;督脉电针可以有效促进移植的嗅鞘细胞在宿主内存活。BACKGROUND：Electro-acupuncture has effects on rehabilitation of spinal cord injury,and cell therapy such as olfactory ensheathing cells（OECs） also exert effects in some patients＇ rehabilitation.Whether both have synergistic effects remains poorly understood.OBJECTIVE：To observe the effects of Du meridian electro-acupuncture and OECs transplantation on content of neurotrophic factor-3 and expression of P75NTR in anterior spinal cord injury rats.METHODS：A total of 40 adult rats were randomly divided into：control group,DU meridian electro-acupuncture group,OECs transplantation group,DU meridian electro-acupuncture ＋ OECs transplantation group.We measured the content of neurotrophic factor-3 and expression of P75NTR by enzyme linked immunosorbent assay at 2 weeks after OECs transplantation and DU meridian electro-acupuncture treatment.RESULTS AND CONCLUSION：The content of NT-3 was highest in DU meridian electro-acupuncture ＋ OECs transplanted group compared with other groups（P 0.05）.There were P75NTR expressions in both OECs transplantation group and DU meridian electro-acupuncture ＋ OECs transplantation group,and the expression was more in DU meridian electro-acupuncture ＋ OECs transplantation group than the OECs transplantation group.These indicated that Du meridian electro-acupuncture and OECs transplantation can elevate the content of neurotrophic factor-3 in anterior spinal cord injury rats.Du meridian electro-acupuncture can improve the survival of transplanted OECs in the host.

关 键 词：督脉电针 嗅鞘细胞移植 前脊髓综合征 神经营养因子3 P75受体 WISTAR大鼠 

分 类 号：R394.2[医药卫生—医学遗传学]