外周血内皮祖细胞数量变化与颅内动脉瘤的生成  

Changes in the number of peripheral blood endothelial progenitor cells and intracranial aneurysm formation

作　　者：徐勇[1,2] 张建宁[1,2] 

机构地区：[1]天津医科大学总医院神经外科 [2]天津医科大学总医院神经病学研究所,天津市300052

出　　处：《中国组织工程研究与临床康复》2011年第6期1083-1087,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30772229)项目名称:脑创伤后血中内皮祖细胞动态变化及意义的研究~~

摘　　要：背景:动脉内膜损伤是动脉瘤发生的始动因素,内皮祖细胞能修复受损的动脉内膜。目的:建立大鼠颅内动脉瘤模型,探讨动脉瘤大鼠内皮祖细胞数量变化及意义。方法:40只SD大鼠随机分为两组,正常组作为正常对照,不给予任何干预。模型组大鼠手术结扎双侧肾动脉后支以及左侧颈总动脉,术后喂食含8%氯化钠鼠粮。于2周,1,2,3个月末测量大鼠内皮祖细胞变化,并与3个月末测量各组大鼠血压及动脉瘤大小,RT-PCR检测willis环相关基因表达。结果与结论:模型组大鼠内皮祖细胞数目于2周后就开始下降,与正常组比较差异有显著性意义(P<0.05),一直持续到3个月末(P<0.01)。模型组大鼠动脉瘤壁基质金属蛋白酶9表达明显高于正常组正常大鼠(P<0.01),而内皮型一氧化氮合酶明显低于正常(P<0.05)。结果提示循环内皮祖细胞数量降低可能是动脉瘤生成的一个重要因素。BACKGROUND：The endothelial dysfunction is a trigger in cerebral aneurysm formation.Endothelial progenitor cells（EPCs） play an important role in repair of the damaged endothelium.OBJECTIVE：To establish rat models of cerebral aneurysm,and to explore changes in the number of EPCs and significance in rats with aneurysm.METHODS：A total of 40 male Sprague-Dawley rats were randomly divided into two groups.The rats in the normal group were left intact.Cerebral aneurysm was induced by ligating the left common carotid artery and posterior branches of the bilateral renal arteries in the model group.After the surgery,the rats were fed a high-salt diet containing 8% sodium chloride.Changes in EPCs of rats were measured at 2 weeks,1,2,3 months.The systemic blood pressure and the size of aneurysm were evaluated at 3 months in each group.Gene expression of willis circle was detected using RT-PCR.RESULTS AND CONCLUSION：EPCs number was significantly decreased in the model group（41±7） at 2 weeks,and there were significant differences between model and normal groups（P 0.05）.The decrease could be detectable at 3 months（P 0.01）.Matrix metalloproteinase-9 expression was significantly greater in the aneurysm wall of rats from the model group compared with normal group（P 0.01）.The endothelial nitric oxide synthase expression was significantly lower in the model group compared with the normal group（P 0.05）.Results suggested that the decrease in the number of circulating EPCs may be a key factor for aneurysm formation.

关 键 词：动脉瘤 内皮祖细胞 动物模型 病因学 内皮型一氧化氮合酶 基质金属蛋白酶 

分 类 号：R394.2[医药卫生—医学遗传学]