蛋白质组学技术鉴定吉兰-巴雷综合征患者血清急性期反应蛋白的表达  

Identifying serum acute phase reactive protein expression in Guillain-Barré syndrome patients using proteomics technology

作　　者：杨国锋[1] 彭立威[1] 纪建国[2] 张锐利[1] 赵景茹[1] 魏晓姗[1] 李培[1] 冯晓[1] 

机构地区：[1]河北医科大学第二医院神经科,河北省石家庄市050000 [2]北京大学生命科学学院蛋白质工程国家重点实验室,北京市100871

出　　处：《中国组织工程研究与临床康复》2011年第7期1249-1252,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30500164):吉兰-巴雷综合征的比较蛋白质组研究;河北省自然科学基金资助项目(C2007000848):吉兰-巴雷综合征的比较蛋白质组研究~~

摘　　要：背景:吉兰-巴雷综合征患者血液中存在与发病有关的抗体、补体和细胞因子,以蛋白质组学技术分离鉴定这些标志蛋白,可为寻找新的药物靶标提供依据。目的:以蛋白质组技术比较吉兰-巴雷综合征患者与正常对照组血清的差异表达蛋白。方法:采集确诊的吉兰-巴雷综合征患者和正常者血清各30例,提取血清蛋白质以固相pH梯度等电聚焦为第一向,SDS-PAGE垂直电泳为第二向进行双向电泳,图象分析软件Imagemaster 2D分析电泳图谱,MALDI-TOF/TOF串联质谱鉴定差异表达蛋白。结果与结论:在吉兰-巴雷综合征患者与正常者中24种蛋白质的表达量显著不同,其中α-2-巨球蛋白,血浆铜蓝蛋白,血清淀粉样P物质,丛生蛋白,抗糜蛋白酶,触珠蛋白,血红素蛋白,α-1-抗胰蛋白酶,血清转铁蛋白等9种蛋白质被鉴定为急性期反应蛋白。结果说明吉兰-巴雷综合征患者血清中急性期反应蛋白表达量发生了明显变化,加深了对吉兰-巴雷综合征发病分子机制的理解。BACKGROUND：Antibody,complement and cytokine related to the pathogensis of Guillain-Barré syndrome（GBS） existed in blood of GBS patients.Identification of these marker proteins can provide evidence for searching new drug targets.OBJECTIVE：To compare differential proteins expression between GBS patients and normal controls by proteomics technology.METHODS：The proteins extracted from GBS patients and normal people were run immobilized pH gradient（IPG） isoelectric focusing electrophoresis as the first dimension,and then run vertical SDS-PAGE as the second dimension.The maps were visualized by silver staining or colloidal coomassive blue and analyzed with ImageMaster 2D Elite software.The proteins of interest were in-gel digested and identified using MALDI-TOF/TOF tandem mass spectrometry.RESULTS AND CONCLUSION：All 24 protein spots were differentially expressed as compared with age-matched control serum,and 9 proteins out of which were members of the acute phase protein,which were identified as α-2-macroglobulin,ceruloplasmi,serum amyloid P-component,Clusterin,α-1-antichymotrypsin,Haptoglobin,Hemopexin,α-1-antitrypsin,Serotransferrin.We got a number of related-proteins of GBS.Some of the proteins are members of the acute phase protein,which are quite useful for discovering the molecular mechanisms of GBS.

关 键 词：吉兰-巴雷综合征 蛋白质组学 血清 双向电泳 质谱 

分 类 号：R318[医药卫生—生物医学工程]