卒中后抑郁患者多巴胺代谢系统COMT和DAT基因多态性分析  

Polymorphisms of catechol-0-methyl transferase gene and dopamine transporter gene indopamine metabolism system of patients with post-stroke depression

作　　者：蔡卫卫[1] 赵连旭[1] 刘振华[1] 陈爱敏[1] 谢惠芳[1] 辛悾[1] 曹琳[1] 贺丽敏[1] 贺荣霓[1] 

机构地区：[1]南方医科大学珠江医院神经内科,广州510282

出　　处：《中华神经医学杂志》2011年第4期351-355,共5页Chinese Journal of Neuromedicine

摘　　要：目的探讨多巴胺递质代谢相关蛋白儿茶酚氧位甲基转移酶（COMT）Vall08／158Met、多巴胺转运体（DAT）40bp可变数目串联重复多态（VNTR）两位点基因多态性与卒中后抑郁（PSD）的关系。方法选择南方医科大学珠江医院神经内科自2010年1月至2010年6月收治的PSD患者68例作为研究组，同期脑卒中后无抑郁患者91例作为对照组，利用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）技术分析患者COMTVall08／158Met、DAT40bpⅥ叮TR位点的基因多态性。结果COMT基因扩增产物分野生型（G／G）、突变纯合（A／A1、杂合型（A／G）3种基因型，删丁基因扩增产物分7／7、9／7、10／7、10／9、10／10、11／10、11／11倍重复7种基因型：2组患者COMT等位基因、基因型分布比较差异均有统计学意义萨5．703，P=0．017；产6．489，P=0．039）。研究组男性患者与对照组男性患者之间比较，COMT等位基因、基因型的分布差异均无统计学意义（P〉0．05）；研究组女性患者与对照组女性患者之间比较，COMT等位基因、基因型的分布差异均有统计学意义（X2=4．610，P=-0．032，X2=6．547，P=-0．024）；研究组与对照组患者DAT等位基因和基因型的总体分布、杂合度比较差异均无统计学意义（P〉0．05）。结论COMTVall08／158Met基因多态性与PSD发生可能有相关性，而DAT40bpVNTR基因多态性与PSD发生无明显相关性。Objective To investigate the association of post-stroke depression （PSD） with gene polymorphisms of catechol-O-methyl transferase （COMT） Val108/158Met and dopamine transporter 40 bp variable number of tandem repeats （VNTR） in dopamine metabolism system. Methods Sixty-eight patients with PSD and 91 patients only suffered from stroke, admitted to our hospital from January 2010 to June 2010, were chosen; the gene polymorphisms ofCOMT Val108/158Met and DAT 40 bp VNTR were analyzed by using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. Results The genotypes of COMT gene amplifications were wild type （G/G）, homozygous mutant type （A/A） and heterozygous type （A/G）; 7 repeated genotypes （7/7, 9/7, 10/7, 10/9, 10/10, 11/10 and 11/11） were noted in the DA T gene amplifications; frequencies of COMT alleles and genotypes were significantly different between the 2 groups 0（X2=-5.703, P=0.017;X2=6.489, P=0.039）. The frequencies of COMT alleles and genotypes were significantly different between the 2 female groups （X2=4.610, P=-0.032; X2=6.547, P=0.024）, but no significant differences were found between the 2 male groups （P〉0.05）. The frequencies and heterozygosity of DAT alleles and genotypes showed no obvious differences between the 2 groups （P〉0.05）. Conclusion The gene polymorphism of COMT Val108/158Met may be associated with PSD, while that ofDAT 40bp VNTR is not.

关 键 词：多巴胺 抑郁 儿茶酚氧位甲基转移酶: 多巴胺转运体 

分 类 号：R749.13[医药卫生—神经病学与精神病学]