环氧化酶-2抑制剂对裸鼠胶质瘤移植瘤Ang基因表达的影响  

Effect of cyclooxygenase-2 inhibitor on angiopoietins gene expression of human glioma xenograftsin nude mice

作　　者：李孝生[1] 刘址忠[2] 王亚东[2] 杜谋选[3] 陈家玉[1] 陈鹏[1] 黄文英 谢艳[1] 林云东[1] 朱贤立[5] 

机构地区：[1]湘南学院附属医院神经外科,郴州423000 [2]胃肠疾病重点实验室,南方医科大学南方医院消化科,广东省510515 [3]南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点研究室,广州510082 [4]第一人民医院高压氧科,郴州市423000 [5]华中科技大学同济医学院附属协和医院神经外科,武汉430022

出　　处：《中华神经医学杂志》2011年第4期369-372,共4页Chinese Journal of Neuromedicine

基　　金：湖南省自然科学基金（09JJ6029）;湖南省卫生厅课题（B2009122）;湖南省教育厅课题[（2008）A065]

摘　　要：目的探讨环氧化酶．2（cox．2）抑制剂尼米舒利（NIM）对裸鼠胶质瘤移植瘤血管生成素似ng）基因表达的影响及意义。方法人胶质瘤SHG44细胞接种于裸鼠皮下，建立裸鼠胶质瘤移植瘤模型，并按随机数字表法分为对照组（灌注等量生理盐水）和NIM治疗组[6mg／（kg·d）]，逆转录PCR技术检测移植瘤组织Ang．1、Ang．2mRNA表达，免疫组织化学染色测定肿瘤组织微血管密度（MVD），并绘制肿瘤生长曲线和计算肿瘤抑制率。结果NIM可有效抑制移植瘤的生长，其抑瘤率为42．03％。NIM治疗组肿瘤组织Ang-2mRNA表达水平（0．2032±0．0185）较对照组（0．6024±0．0289）明显降低，差异有统计学意义（P〈0．05）；Ang-1mRNA表达水平无明显改变，差异无统计学意义（胗0．05）：Ang-2／Ang-1mRNA比值下降（0．5825±0．0621vs1．5847±0．1948）．差异有统计学意义（P〈0．05）。NIM治疗组肿瘤组织MVD较对照组明显下降，差异有统计学意义（P〈0．05）。结论COX-2抑制剂NIM可下调An-2基因表达，改变Ang-2／Ang-1mRNA比值，抑制肿瘤生长。Objective To investigate the effect of nimesulide （N1M）, a selective cyclooxygenase-2 （COX-2） inhibitor, on angiopoietins （A ng） gene expression of human glioma xenografts in nude mice and its significance. Methods Human SHG44 glioma cells were inoculated subcutaneously in 16 nude mice to establish xenograft models, and then these mouse models were randomly divided into NIM treatment group and control group. NIM （6 mg/kg） and saline were poured into the stomachs of the mice in each group, respectively, once daily for 35 d. The mRNA expressions of A rig-1 gene and A ng-2 gene in the xenografts were determined by RT-PCR. Microvessel density （MVD） in the xenogral^s was assessed by immunohistochemical technique. The tumor growth curve was drawn and the inhibition ratio of tumor growth was calculated. Results NIM could significantly inhibit the glioma xenografts growth with its inhibition rate reaching 42.03%. The mRNA expression of A rig-2 gene in NIM treatment group （0.2032±0.0185） was significantly lower than that in control group （0.6024±0.0289, P〈0.05）, but that ofAng-1 gene showed no significant changes; therefore, the mRNA ratio of Ang-2/Ang-1 genes was decreased （0.5825±0.0621 vs. 1.5847±0.1948,P〈0.05）. MVD in the xenografts of the NIM treatment group was significantly lower than that in the control group （P〈0.05）. Conclusion NIM, by down-regulating the mRNA expression of A rig-2 gene and changing the mRNA ratio of Aig-2/ Aig-1 genes, can inhibit the tumor growth

关 键 词：环氧化酶-2 神经胶质瘤 血管生成素 

分 类 号：R735.7[医药卫生—肿瘤]