转化生长因子β1诱导星形胶质细胞产生Jagged1的初步探讨  

A preliminary study on the genesis of Jagged1 mRNA in astrocyte induced by TGF-β1

作　　者：高霞[1] 曾彦英[1] 顾冰洁[2] 马玉苹[1] 王松[2] 季晓辉[2] 丁国宪[1] 刘莉[1] 陈伟贤[1] 

机构地区：[1]南京医科大学第一附属医院老年医学科,210029 [2]南京医科大学免疫与微生物教研室

出　　处：《江苏医药》2011年第7期755-757,共3页Jiangsu Medical Journal

基　　金：江苏省卫生厅"135"工程重点学科基金(200131);江苏省教育厅自然科学基金(20081305)

摘　　要：目的探讨转化生长因子β1(TGF-β1)对激活星形胶质细胞产生Jagged1基因的影响。方法对脂多糖(LPS)激活的星形胶质细胞,给予TGF-β1培养24 h,提取星形胶质细胞mRNA,观察各个时间点(0、6、12及24 h)Jagged1 mRNA的水平。结果 TGF-β1促进星形胶质细胞产生Jagged1 mRNA,并随培养时间的延长,星形胶质细胞中Jagged1 mRNA水平逐渐衰减(P<0.05)。结论 TGF-β1能使激活的星形胶质细胞产生Jagged1 mRNA,可能通过Jagged1/Notch通路的激活导致髓鞘修复障碍。Objective To investigate the effect of TGF-β1 on the genesis of Jagged1 mRNA in astrocyte.Methods After the astrocyte activated by LPS was cultivated with TGF-β1 for 24 hours,the Jagged1 mRNA was extracted.The expression of Jagged1 mRNA was evaluated at 0,6,12 and 24 h.Results TGF-β1 induced the expression of Jagged1 mRNA in astrocyte.As culture duration extended,the expression of Jagged1 mRNA was attenuated significantly（P0.05）.Conclusion TGF-β1 can induce the expression of Jagged1 mRNA in the LPS-activated astrocyte,which may inhibit the repair of myelin sheath through the activation of Jagged1/Notch1 pathway.

关 键 词：星形胶质细胞 Jagged1基因 转化生长因子Β1 

分 类 号：R741[医药卫生—神经病学与精神病学]