非酒精性脂肪性肝病兔肾组织硫化氢与一氧化氮变化  

Changes of Nitrogen Oxide and Hydrogen Sulfide in Rabbit Kidney Tissue with Nonalcoholic Fatty Liver Disease

作　　者：谭华炳[1] 雷飞飞[1] 王一平[1] 胡小林[2] 朱德文[2] 任永生[3] 

机构地区：[1]湖北医药学院附属人民医院感染性疾病科,湖北省十堰市442000 [2]湖北医药学院第三临床学院 [3]湖北医药学院基础医学院生理学教研室

出　　处：《中华全科医学》2011年第5期672-673,694,F0003,共4页Chinese Journal of General Practice

基　　金：湖北省教育厅科技项目(D20102104);湖北省卫生厅科研项目(JX5B75);湖北省十堰市科技局科技项目(2010st33);湖北医药学院学生科研项目(2010XSA19)

摘　　要：目的观察非酒精性脂肪性肝病（NAFLD）兔肾组织硫化氢（H2S）、一氧化氮（NO）浓度,探讨H2S、NO在NAFLD肾损害中的作用。方法将40只日本大耳白兔数字法随机分为重度NAFLD组（重度组）、轻度NAFLD组（轻度组）、对照组。重度组予高脂饲料160 g/（兔·d）,轻度组予高脂饲料80 g/（兔.d）＋普通饲料80 g/（兔.d）,对照组予普通饲料160 g/（兔.d）。饲养13周。肾组织匀浆检测NO、H2S浓度。免疫组化染色检测肾组织α-平滑肌肌动蛋白（α-SMA）表达。HE染色,光镜观察肝组织病理学。结果①NO浓度：重度组[（16.24±7.8）μmol/g pro]、轻度组[（22.54±5.4）μmol/g pro]与对照组[（27.32±9.3）μmol/g pro]比较,差异有统计学意义（P〈0.01,P〈0.05）,重度组与轻度组比较差异有统计学意义（P〈0.05）。②H2S浓度：重度组、轻度组肾组织H2S与对照组相比明显下降（P〈0.01）,重度组与轻度组相比差异无统计学意义（P（0.05）。③α-SMA检测：对照组α-SMA存在于血管壁;重度NAFLD组除血管壁外,肾小球系膜区及肾间质均有表达;轻度组较重度组α-SMA表达减轻。④肝脏病理学：重度组肝脏呈重度NAFLD,轻度组呈轻度~中度NAFLD。结论在NAFLD发生、发展过程中肾组织H2S、NO下降,NO、H2S可能参与NAFLD肾损害过程。Objective To observe the changes of Nitric oxide（NO） and Hydrogen sulfide（H2S） in kidney tissue of the rabbit with Nonalcoholic fatty liver disease（NAFLD）,and to investigate effect of H2S and NO in injured kidney of NAFLD.Methods 40 Japanese white rabbits were randomly divided into severe group with NAFLD,mild group with NAFLD,blank group.Severe group was given high fat diet 160 g/rabbit.day,mild group were given high fat diet 80 g /rabbit.day and normal diet 80 g/rabbit.day,blank group was given normal diet 160 g/rabbit.day.The raising cycle was 13 weeks.The Density of NO and H2S in kidney tissue were tested.Immuno-histochemistry staining was used to detect the expression of α-SMA in Rabbit Kidney Tissue.HE staining,pathological changes of liver tissue were observed with the aid of microscope.Results ①The level of NO：Severe group of NO in kidney tissue was（16.24±7.8）μmol/g.pro,mild group of NO in kidney tissue was（22.54±5.40）μmol/g.pro,there was a significant difference compared with blank group（P〈0.01,P〈0.05）,there was a significant difference between severe group and mild group（P〈0.05）.②The level of H2S：severe group and mild group significantly decreased in liver tissue,there was a significant difference compared with blank group（P〈0.01）,there was no significant difference between Severe group and mild group（P﹥0.05）.③Detected α-SMA： The blank group rabbit kidney of α-SMA mainly existed in the blood vessel walls,mesangial area and renal stroma also expressed α-SMA except the blood vessel walls in Severe NAFLD group,the expression of α-SMA in mild group decreased compared with mild group.④Liver pathology： severe group of moderate was severe NAFLD,mild group was mild to moderate NAFLD,blank group was normal liver.Conclusion H2S and NO participated the occurrence and development of NAFLD,it was the future direction through interventing the NO and H2S possibly.The levels of H2S and NO decreased in the process of the occurrence and development of

关 键 词：非酒精性脂肪性肝病 肾组织 硫化氢 一氧化氮 Α平滑肌肌动蛋白 动物 

分 类 号：R575[医药卫生—消化系统]