转化生长因子β3在小鼠牙胚钟状晚期以后发育中的表达和分布  

Expression and distribution of transforming growth factor β3 in the mouse tooth germ during development after advanced bell stage

作　　者：阙国鹰[1] 李恒[2] 张磊[2] 邹莉[2] 吴英[1] 

机构地区：[1]中南大学湘雅医院口腔科,长沙410008 [2]中南大学口腔医学院,长沙410008

出　　处：《中南大学学报（医学版）》2011年第3期254-257,共4页Journal of Central South University ：Medical Science

基　　金：中南大学大学生创新性试验计划(YA07053)~~

摘　　要：目的:观察转化生长因子β3(transforming growth factorβ3,TGF-β3)在小鼠牙胚钟状晚期以后发育中的表达分布,探讨其在小鼠牙胚钟状晚期以后发育中的作用。方法:取近交系BALB/C小鼠出生后4 d(4 days postnatal,4dpn)、11 d(11dpn)及18 d(18dpn)的牙胚,常规固定、脱钙、包埋、切片,用SP法免疫组织化学染色技术检测TGF-β3在各牙胚中的表达。结果:4dpn牙胚成釉细胞层、成牙本质细胞层呈强阳性表达,牙乳头周边区域细胞呈阳性表达,中间区域呈弱阳性表达;11dpn牙胚成釉细胞层呈弱阳性表达,成牙本质细胞层、牙乳头细胞呈阴性表达;18dpn牙胚牙周膜中的血管壁及周围呈强阳性表达,牙本质、牙骨质、牙周膜成纤维细胞呈阴性表达。结论:TGF-β3在小鼠牙胚钟状晚期以后发育过程中的表达具有一定的时空分布性,可能在牙胚发育中有一定的调控作用,这种作用可能随着牙胚的发育而逐渐减弱。Objective To observe the expression and distribution of transforming growth factor β3（TGF-β3） in the mouse tooth germ after advanced bell stage,and to discuss the role of TGF-β3 during the development of tooth germs.Methods BALB/C＇s mouse tooth germs at 4,11,and 18 days postnatal（4dpn,11dpn,and 18dpn） were collected and processed for routine fixation,decalcification,embedding,and slicing.The expression of TGF-β3 was detected by immunohistochemisty.Results As to 4dpn tooth germ： Positive expression of TGF-β3 was found in enameloblasts,odontoblasts,ambitus of dental pupilla,with weak positive expression in the intermedial of dental papilla.As to 11dpn tooth germ： Positive expression was seen in enameloblasts,with negative expression in odontoblasts and dental papilla.As to 18dpn tooth,positive expression of TGF-β3 was showed in the vessel wall and its surrounding,with negative expression in other areas.Conclusion The distribution of TGF-β3 expression showed a time-space characteristic during the mouse tooth germ development after advanced bell stage,which may exert a regulatory effect on tooth development and this effect is gradually getting weak with the development of tooth germs.

关 键 词：牙胚 转化生长因子Β3 免疫组织化学 

分 类 号：R780.2[医药卫生—口腔医学]