巴西橡胶树胶乳化学成分的GC-MS分析  

Analysis of Chemical Components of the Latex from Hevea brasiliensis by GC-MS

作　　者：梅志刚[1,2] 刘实忠[2] 校现周[2] 罗世巧[2] 杨文凤[2] 牛静明[1,2] 黄荣辉[1,2] 

机构地区：[1]海南大学园艺园林学院,海南儋州571737 [2]中国热带农业科学院橡胶研究所,海南儋州571737

出　　处：《热带作物学报》2011年第2期315-319,共5页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院橡胶研究所基本科研业务专项资金项目(No.XJSYWFZX2009-15)

摘　　要：采用气相色谱-质谱联用(GC-MS)技术,对巴西橡胶树胶乳氯仿、乙酸乙酯萃取物中的化学成分进行分析测定,结合计算机检索技术对其成分进行结构鉴定,应用色谱峰面积归一化法计算各成分的相对百分含量。结果表明,氯仿萃取物中共鉴定出33个化学成分,占总量的78.90%。乙酸乙酯萃取物中共鉴定出46个化学成分,占总量的90.15%。其中,τ-杜松醇、(Z)-金合欢醇、角鲨烯、环阿屯醇、24-亚甲基环木菠萝醇等萜类化合物及γ-谷甾醇、β-豆甾醇、菜油甾醇、异岩藻甾醇等植物甾醇在橡胶生物合成途径甲羟戊酸途径中作为中间体或终产物。除24-亚甲基环木菠萝醇以外,几种萜类和甾醇都具有一定药用作用,尤其是角鲨烯,具有抗癌、抗氧化、预防心血管疾病等生理活性,目前已成为天然药物研究的热点。Chemical constituents of chloroform and ethyl acetate extract of the latex from Hevea brasiliensis were analyzed by gas chromatography-mass spectrometry（GC-MC）,and identified by their mass spectra,their relative percentage was calculated from the GC peak areas.Thirty-three compounds were identified from chloroform extract,accounting for 78.90%of the total detected constituents.Forty-six compounds were identified from ethyl aectate extract,accounting for 90.15%of the total detected constituents.Five terpenes τ-cadinol,（Z）-farnesol,squalene,cycloartenol,and 24-methylenecycloartanol and four sterols γ-sitosterol,β-stigmasterol,campesterol,isofucosterol play a role as the intermediate or the end product in mevalonate pathway which is the pathway of rubber biosynthesis.Those terpenes and sterols have certain medicinal function except 24-methylenecycloartanol.Especially squalene,has become the research focus in natural medicine because of its anticarcinogenic,antioxidant and anti-infection activities.

关 键 词：胶乳 气相色谱-质谱联用技术 化学成分 

分 类 号：Q591.1[生物学—生物化学]