广西非小细胞肺癌组织K-ras癌基因mRNA表达的研究  

Study on mRNA Expression of K-ras Oncogene in Non-small Cell Lung Cancer in Guangxi

作　　者：钟炜祥[1] 陈铭伍[1] 冼磊[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,南宁市530021

出　　处：《广西医学》2011年第3期264-267,共4页Guangxi Medical Journal

基　　金：广西科学研究与技术开发计划项目(桂科攻10124001A-44);广西大型仪器协作共用专项资金项目

摘　　要：目的探讨广西非小细胞肺癌(NSCLC)组织K-ras癌基因mRNA表达及其与肺癌各临床病理特征的关系。方法应用反转录-聚合酶链反应(RT-PCR)法检测86例广西的NSCLC患者癌组织(肺癌组)和31例癌旁组织(肺组织组)中K-ras癌基因mRNA表达。结果肺癌组K-ras癌基因mRNA表达率为80.2%(69/86),显著高于肺组织组的51.6%(16/31),差异有统计学意义(P<0.01);K-ras癌基因mRNA表达与年龄、性别、民族、吸烟史、病理类型、肿瘤细胞分化程度、TNM分期及淋巴结状态等均无统计学相关(P>0.05)。结论 K-ras癌基因的过度表达参与了NSCLC的发生,但与广西NSCLC的发展及预后无关。Objective To explore the mRNA expression of K-ras oncogene and the relationship between the expression of K-ras oncogene and the clinicopathological characteristics of non-small cell lung cancer（NSCLC）in Guangxi.Methods The mRNA expression of K-ras oncogene was detected by reverse transcription polymerase chain reaction（RT-PCR） in 86 cases of NSCLC tissues and 31 cases of adjacent normal tissues.Results The mRNA expression rate of K-ras oncogene in NSCLC tissues and adjacent normal tissues were 80.2% and 51.6%,respectively,the former was significantly higher than the latter（P0.01）.However,the mRNA expression of K-ras oncogene in NSCLC was not correlated with age,sex,nationality,smoking history,pathological histologicol styles,degree of cell differentiation tumor,TNM stages and lymph node metastasis（P0.05）.Conclusion The over-expression of K-ras oncogene,which was not asscociated with the development process and prognosis,involved in the occurrence in NSCLC.

关 键 词：非小细胞肺癌 K-RAS 癌基因 反转录-聚合酶链反应 

分 类 号：R734.2[医药卫生—肿瘤]