小麦抗氧化能力对Cs^+富集响应的研究  

RESPONSES OF ANTIOXIDANT CAPACITY TO Cs^+ BIOCONCENTRATION IN WHEAT

作　　者：安冰[1,2] 唐运来[1,2] 陈梅[1,2] 敖嘉[1,2] 王丹[1,2] 

机构地区：[1]西南科技大学生命科学与工程学院,四川绵阳621000 [2]西南科技大学核废物与环境安全国防重点学科实验室,四川绵阳621000

出　　处：《核农学报》2011年第2期348-352,共5页Journal of Nuclear Agricultural Sciences

基　　金：西南科技大学国防重点项目(07XJGZB05);四川省科技厅应用基础研究项目(2009JY0144);农业部核农学重点开放实验室基金项目(KYJD09017)

摘　　要：以小麦(Triticum aestivum L.)为材料,在三叶期用不同浓度Cs+(CsCl)进行处理,处理浓度分别为0、0.5、1、5、10和20mmol.L-1,处理7、14、21和28d后分别取样,研究小麦对不同浓度Cs+的富集差异,并探讨Cs+对小麦幼苗抗氧化能力的影响。结果表明,小麦幼苗中Cs+含量与处理浓度和处理时间呈显著正相关,根是主要的富集器官,其次为茎;在20mmol.L-1处理28d后,根的Cs+含量分别是叶和茎的3.3和1.7倍;根、茎、叶Cs+含量分别是0.5mmol.L-1处理浓度的51.3、64.2和43.3倍。所有处理时期根茎叶转运系数和富集系数与处理时间呈显著正相关,均为S/R>L/R和R/S>S/S>L/S。对抗氧化指标的分析发现,随着Cs+浓度的增加和处理时间的延长,小麦幼苗POD和CAT活性均表现为先应激性上升后下降的动态变化,MDA和H2O2含量均呈上升趋势,且与处理时间均呈显著正相关。这表明POD和CAT清除过氧化物的能力是有限的,高浓度时均受到一定程度的抑制,抗氧化体系在一定程度上受到破坏。The present study aimed to analyze the biological effects induced by bioaccumulation of cesium in Triticum aestivum L..Seedlings of trefoil stage were treated with 0,0.5,1,5,10 and 20mmol.L-1Cs ＋ in Hoagland solution.Following 7,14,21 and 28 days’exposure,plants were monitored for cesium uptake and parameters involved in the antioxidative defense（including activity of POD and CAT,H2O2 and MDA content）.Cesium contents were up to 3.3-fold and 1.7-fold higher in roots compared with leaves and stems（following 28 days’exposure to 20mmol.L-1 Cs ＋）,and cesium contents of roots,stems and leaves were up to 51.3-fold、64.2-fold and 43.3-fold higher than exposure to 0.5mmol.L-1 Cs ＋ r,espectively.The results showed that the roots was the highest Cs ＋ accumulation organs.Both of transfer factor and bioconcentration factor showed significantly positive relationship with treatment time,which were S /R L /R and R /S S /S L /S entirely.For antioxidative parameters analysis,the activity of POD and CAT increased at first,and then declined following the increasing of Cs ＋ concentration and the treatment time;and H2O2 and MDA content continuously increased with the increasing of the Cs ＋ concentration and the treatment time.The results showed that the capability of clearing up the harmful peroxide for POD and CAT were limited,then POD and CAT were inhibited under higher Cs ＋ concentration,and antioxidant system were impaired in some way.

关 键 词：小麦 Cs+ 富集系数 抗氧化能力 

分 类 号：S512.1[农业科学—作物学]