快中子辐射诱变对绿色木霉产纤维素酶的影响  

Effect of Fast Neutron Irradiation on Production of Cellulase from Trichoderma viride

作　　者：陈光[1] 徐杨[1] 孙旸[1] 刘洁心[1] 王香琪[1] 

机构地区：[1]吉林农业大学生命科学学院,长春130118

出　　处：《吉林农业大学学报》2011年第2期177-180,共4页Journal of Jilin Agricultural University

基　　金：吉林省科技发展重点项目(20100226)

摘　　要：利用快中子对绿色木霉AS3.3711进行辐照,辐照剂量为0.6-4.8 Gy。研究不同剂量快中子对绿色木霉孢子致死率和遗传稳定性的影响,得到了提高纤维素酶活性的菌株。结果表明：辐照剂量在0.6~4.8 Gy范围内绿色木霉的致死率呈逐渐上升趋势,辐照剂量为1.2 Gy时,绿色木霉的致死率达到80.0%,此突变株的羧甲基纤维素酶活（CMC酶活）可达914.4 U/mL,比出发菌株提高了30倍,滤纸酶活（FPA酶活）为633.63 U/mL,比出发菌株提高了4倍。The effect of deuterium ion bombardment majority targeted to produce 14Mev fast neutron irradiation,with dosage 0.6—4.8Gy,on production of cellulose from Trichoderma viride AS3.3711 was studied.The death rate was in a rising trend when irradiation dosage was 0.6—4.8 Gy.The results indicated the death rate of spores reached 80.0% when radiation dosage was 1.2 Gy.It was shown that one positive mutant had significant difference compared to the original with paired samples test（t-test）.The filter paper activity of cellulase of strain Fn10-1 reached 633.63 U/mL,with 4 times enhanced,compared with the initial strain.And the CMC activity of cellulase of strain Fn10-1 reached 914.4 U/mL,with 30 times enhanced,compared with the initial strain.

关 键 词：绿色木霉 纤维素酶 快中子 

分 类 号：Q691.8[生物学—生物物理学] Q939.5