球形红细菌好氧降解氯代苯研究  

Study on aerobic degradation of chlorobenzene by Rhodobacter sphaeroides

作　　者：王玉芬[1,2] 张肇铭[2,3] 胡筱敏[2] 贡俊[1] 

机构地区：[1]山西财经大学环境经济学院,太原030006 [2]东北大学资源环境与土木工程学院,沈阳110004 [3]山西大学生命科学与技术学院,太原030006

出　　处：《环境工程学报》2011年第5期1187-1193,共7页Chinese Journal of Environmental Engineering

基　　金：国家科技攻关项目(2001BA540C);山西省高校科技研发项目(20081021)

摘　　要：采用光合细菌球形红细菌在好氧条件下对氯代苯进行生物降解。结果表明,氯代苯不能作为球形红细菌生长的惟一碳源和能源,球形红细菌好氧降解氯代苯是在适宜碳源存在下以共代谢的方式进行;根据分析细胞提取液中双加氧酶活性和代谢中间产物,推断出其降解机制为邻位裂解途径,按先开环再脱氯过程进行,邻苯二酚1,2-双加氧酶的活性可以通过氯代苯的诱导显著提高。当氯代苯初始质量浓度为100 mg/L时,好氧降解的最适宜条件为苹果酸质量浓度1.5g/L、非光照、pH 7。Chlorobenzene was biodegraded by photosynthetic bacteria Rhodobacter sphaeroides under aerobic condition.The results suggested that chlorobenzene could not be taken as sole carbon and energy sources of Rhodobacter sphaeroides.The aerobic degradation of chlorobenzene by Rhodobacter sphaeroides was carried out with the cometabolism way in the presence of the suitable carbon source.It was proposed that the degradation mechanism was an ortho clevage pathway,by analyzing the activities of dioxygenase enzymes in cell extracts and metabolic intermediates.The degradation ways were first opening chlorobenzene ring,then dechlorination.The activity of catechol-1,2dioxygenase could be improved markedly by chlorobenzene induction.When the initial concentration of chlorobenzene was 100 mg/L,the most suitable conditions of aerobic degradation were malic acid concentration of 1.5 g/L,dark,and pH of 7.

关 键 词：光合细菌 球形红细菌 好氧降解 氯代苯 

分 类 号：X172[环境科学与工程—环境科学]