Ⅳ型胶原酶尾壳核内注射致中重度脑出血大鼠模型的建立  

Establishment of moderate to severe cerebral hemorrhage model by injecting type Ⅳ collagenase into caudate putamen nuelei

作　　者：王坤[1] 张少军[1] 

机构地区：[1]蚌埠医学院第一附属医院神经外科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2011年第4期339-341,共3页Journal of Bengbu Medical College

摘　　要：目的:通过Ⅳ型胶原酶+肝素钠尾壳核内注射建立一种稳定的中重度脑出血大鼠模型。方法:66只SD大鼠随机分成Ⅳ型胶原酶0.4 U组(A组,12只)、Ⅳ型胶原酶0.6 U组(B组,42只)及Ⅳ型胶原酶0.8 U组(C组,12只),3组同时均配伍肝素4 U。制作脑出血模型后A组24、72 h,B组4、12、24、48、72 h以及5、7 d,C组24、72 h对大鼠进行神经功能学评分检测肢体功能缺损情况,通过多媒体病理图像分析仪测定脑血肿体积大小及水肿带体积大小。结果:24、72 h血肿体积、水肿体积及神经功能评分3组间差异均有统计学意义(P<0.01)。A组血肿体积小,较弥散,水肿带较窄,神经功能缺损较轻;B、C组血肿体积大,边界清楚,水肿带较宽,神经功能缺损重;但C组病死率高达37%,B组病死率较低,<6%。结论:0.6 UⅣ型胶原酶+4 U肝素尾壳核内注射可以诱导建立稳定的中重度脑出血大鼠模型,模型肢体功能缺损明显,存活率高,能作为促进脑出血大鼠神经功能恢复研究的可靠应用平台。Objective：To establish moderate to severe intracerebral hemorrhage models by injecting type Ⅳ collagenase and heparin into the caudate putamen nuclei of rats.Methods：A total of 66 SD rats were randomly divided into Ⅳ collagenase 0.4 U ＋ heparin 4 U injection group（group A,12 rats）,Ⅳ collagenase 0.6 U ＋ heparin 4 U injection group（group,B 42 rats）and Ⅳ collagenase 0.8 U＋heparin 4 U injection group（group C,12 rats）.The neurological behavior scores of the rats in group A and group C were assessed at 24 and 72 hour after the injection,and those of group B were assessed at 4,12,24,48,72 hour,5 and 7days after the injection.The volume of hematoma and edema was detected by multimedia pathological imaging analysis system.Results：The scores of neurological behavior,the volume of hematoma and edema had obvious difference among the three groups（P0.01）.The hematoma in group A was small and diffused,the edema strip was narrow,and the neurological deficit was light compared with those of group B and C.However,the mortality after injection was 37% in group C,which was much higher than that in the other two groups.Conclusions：Intracerebral hemorrhage models can be established by injecting 0.6 U Ⅳ collagenase and 4 U heparin into the caudate putamen nuclei of rats.The models have obvious neurological deficits and a high persistence,which may be used for the study of intracerebral hemorrhage.

关 键 词：脑出血 疾病模型 动物 大鼠 胶原酶类 肝素钠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]