致病疫霉拮抗放线菌NB8发酵条件的优化  

Optimization of fermentation conditions of Streptomyces albus NB8 against the growth of Phytophthora infestans

作　　者：惠娇[1] 蒋继志[1] 鲍雪[1] 王芳[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北农业大学学报》2011年第2期86-89,共4页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金项目(C2011201003)

摘　　要：为提高放线菌NB8(Streptomyces albus)菌株对致病疫霉(Phytophthora infestans)的抑制效率,本试验采用滤纸片法以单因子试验对拮抗放线菌NB8菌株的发酵条件进行优化。结果表明优化后的最佳发酵条件为:将NB8菌株经高氏一号液体培养5 d的种子液,以125 mL/250 mL的装液量和1.5%的接种量,在初始pH7.0、34℃、50 r/min及12 h黑暗/12 h光照下培养10 d所得发酵液对致病疫霉的抑制作用最强,抑菌率达到81%以上,比优化前提高了约20%。结果表明NB8菌株在控制马铃薯晚疫病方面有较大的应用潜力。In order to enhance the inhibitory effect of substances in fermentation liquid of Streptomyces albus NB8 strain against the growth of Phytophthora infestans,the fermentation conditions of NB8 strain were investigated by single-factor experiment.The results showed that optimal fermentation conditions were as follows：seed liquid cultured in Gause 1 medium after 5 d was inoculated with a volume of 125 mL/250 mL and amount of 1.5% into same medium,then was cultured 10 d as the initial pH7.0,34 ℃,50 r/min,12 h dark/12 h light.And the inhibitory rate reached 81% under the optimal fermentation conditions,which increased by 20% in comparison with pre-optimization.The findings indicate that NB8 strain has a great potential in control potato late blight in future.

关 键 词：致病疫霉 拮抗放线菌 发酵条件 

分 类 号：S436.412.12[农业科学—农业昆虫与害虫防治]