嗜麦芽寡养单胞菌D2外泌蛋白酶抗肿瘤活性初步探讨  

PRELIMINARY STUDY ON THE ANTI-TUMOR ACTIVITY OF STRAIN D2 PROTEASE OBTAINED FROM THE DIGESTIVE TRACT OF PERINEREIS AIBUHITENSIS GRUBE

作　　者：姜光域[1] 李玲[1] 闫志勇[1] 钱冬萌[1] 赵巍[1] 王斌[1] 

机构地区：[1]青岛大学医学院青岛市医药生物技术重点实验室,山东青岛266071

出　　处：《青岛大学医学院学报》2011年第1期5-7,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家重大基础研究前期专项(2004CCA024000);山东省科技发展计划项目(2004GG2205136);青岛市科技专项(05-1-P-10);泰山学者工程资助

摘　　要：目的研究沙蚕消化道产蛋白酶菌D2株胞外蛋白酶(D2蛋白酶)的抗肿瘤活性。方法选用鼠乳癌细胞EMT6作为靶细胞,经不同浓度的D2蛋白酶作用不同时间,采用MTT法对体外培养靶细胞进行细胞增殖抑制率检测,并与阳性对照组(紫杉醇组)和正常细胞对照组(人胚肺细胞组)比较。结果 D2蛋白酶对鼠乳癌细胞EMT6增殖有抑制作用,且呈现浓度及时间效应关系。结论 D2蛋白酶具有一定的抗肿瘤活性。Objective To study the antineoplasmic activity of strain D2 protease obtained from the digestive tract of Perinereis aibuhitensis Grube.Methods Different dosages of protease D2 were used for culturing the EMT6.MTT assay was performed to evaluate the inhibitory effect of protease D2 on the cell proliferation,and compared with positive control group（paclitaxel group） and normal-cell control group（human-embryonic-lung-cell group）.Results Protease D2 inhibited the proliferation of EMT6 cells,which showed a time and concentration relationship.Conclusion Protease D2 possesses a certain antineoplasmic activity.

关 键 词：肽水解酶类 药物筛选试验 抗肿瘤 乳腺肿瘤 

分 类 号：R737.9[医药卫生—肿瘤]