海南汉族乙型肝炎病毒基因型与BCP区和前C区基因突变的关系  

Corelation between hepatitis B virus genotypes and BCP/PreC gene mutations in Hainan Island

作　　者：曾俊涛[1] 陈静[1] 曾仕平[1] 钟良宝[1] 

机构地区：[1]海南医学院附属医院,海口570102

出　　处：《山东医药》2011年第13期4-6,共3页Shandong Medical Journal

基　　金：海南省卫生厅基金项目(琼卫-200548;200852)

摘　　要：目的探讨海南汉族乙型肝炎病毒(HBV)基因型与前C区G1896A和BCP区A1762T/G1764A基因突变的关系。方法采用RT-PCR方法检测乙型肝炎患者的HBV基因型,PCR方法扩增包含C启动子和前C区基因核苷酸(nt1643-nt2112),对PCR产物进行DNA测序。结果基因型C的BCP区A1762T/G1764A突变率(58.82%)显著地高于基因型B(10.53%)(P<0.05)。基因型C G1896A突变率为29.41%,基因型B G1896A突变率为47.37%,两者比较差异无统计学意义(P>0.05)。结论不同基因型HBV致病能力可能与病毒基因组BCP区A1762T/G1764A突变率的不同有关,而与前C区G1896A突变无关。Objective To investigate the relation between hepatitis B genotype and pre-eore G1896A mutation and BCP A1762T/G1764A mutation in patients with chronic hepatitis B in Hainan. Methods HBV genotype was determined by real time fluorimetry polymerase chain reaction. Promoter C and ribonueleotide in pre-eore C zone（nt1643-nt2112）. DNA sequencing was used to PCR products. Results BCP A1762T/G1764A mutation frequency in patients infected with genotype C （ 58.82% ） was higher than that of those with genotype B （ 10.53% ） （ P 〈 0.05 ） ; G1896A mutation frequency was not significant difference between genotype C group （29.41%） and genotype B group （47.37%）. Conclusions Pathogeneeity of different HBV genotypes may relate to BCP A1762T/G1764A mutation frequency and have no relation with G1896A mutation.

关 键 词：肝炎病毒 乙型 基因型 前C区 基本核心启动子 突变 

分 类 号：R587.1[医药卫生—内分泌]