CXCR7-shRNA慢病毒表达载体对人结肠癌细胞生物学特性的影响  

Effects of lentiviral vector loaded CXCR7-shRNA on biological characteristics of human colon cancer cell

作　　者：王红鲜[1] 陶霖玉[1] 齐柯[1] 向红[1] 张好云[1] 冯铎[1] 孔恒[1] 林秋生[1] 陈天文[1] 

机构地区：[1]广东医学院附属南山医院,广东深圳518052

出　　处：《山东医药》2011年第13期18-20,共3页Shandong Medical Journal

摘　　要：目的探讨CXCR7-shRNA慢病毒表达载体靶向抑制CXCR7的表达对人结肠癌细胞增殖、迁移活性的影响。方法将人结肠癌细胞HT-29分为实验组、阴性对照组和空白对照组;实验组加入CXCR7-shRNA慢病毒表达载体;阴性对照组加入CXCR7-shRNA慢病毒阴性对照;空白对照组不做任何处理。W estrn b lot方法测定各组细胞CXCR7蛋白表达水平。细胞活性实验、Transwell细胞迁移实验检测各组细胞的增殖和迁移活力。结果实验组CXCR7 mRNA相对表达水平低于阴性对照组和空白对照组(P均<0.05);细胞活性实验显示实验组细胞生长曲线较平缓,细胞生长速度较阴性对照组和空白对照组显著降低(P均<0.05);Transwell细胞迁移实验显示实验组穿透滤过膜细胞数量较阴性对照组和空白对照组明显减少(P均<0.05)。结论靶向沉默CXCR7的表达抑制结肠癌细胞增殖和迁移活性。Objective To study the proliferative and migratory activities of human colon cancer cell after CXCRT-targeting inhibition by lentiviral vector loaded CXCRT-shRNA. Methods Human colon cancer cells HT-29 were divided into 3 groups： experimental group, negative control group and blank control group. The experimental group was administrated with LV-CXCR7shRNA, negative control group with LV-shRNA negative control and blank control group without the any. CXCR7 protein of every group were detected using Westrn blot. The proliferative and migratory activities of every group were detected using cell active assay and Transwell cell migratory assay. Results CXCR7 protein was lower expression in experimental group than the negative control group and blank control group （ P 〈 0.05）. The cell growth curve of experimental group was flatter and the level of cell growth was significantly lower than the negative control group and blank control group （ P 〈 0.05 ）. The cell growth curve of negative control group was lower than that of blank control group. Transwell cell migratory assay： the cell populations of penetrating the filtrative membrane in experimental group were significantly lower than that of negative control group（ P 〈 0.05 ）. Conclusions Gene silence of CXCR7-targeting suppresses the proliferative and migratory activities of colon cancer cell.

关 键 词：结肠肿瘤 CXCR7-shRNA 细胞活性实验 细胞迁移实验 

分 类 号：R737.35[医药卫生—肿瘤]