Analysis of Protein and Isoenzyme of LiLum davidii var.unicolor,Lilium Asiatic Hybrids and Their Hybrid F_1  

兰州百合与亚洲百合及其杂种F_1的蛋白质和同工酶分析(英文)

作　　者：李雪[1] 杨彩玲[1] 张鸣[1] 霍彩霞[1] 朱彦荣[1] 

机构地区：[1]兰州城市学院化学与环境科学学院,甘肃兰州730070

出　　处：《Agricultural Science & Technology》2011年第1期54-57,共4页农业科学与技术（英文版）

摘　　要：[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.[目的]为选配亲本组合及杂种早期鉴定提供理论依据。[方法]采用聚丙烯酰胺凝胶电泳技术,对兰州百合与亚洲百合及杂交后代的可溶性蛋白质和过氧化物酶进行分析。[结果]以兰州百合为亲本的杂交后代的蛋白质谱中不仅出现了与亲本同源但比亲本染色加深的带,且出现了亲本中不存在的新带。杂种F1过氧化物酶谱主要表现为亲本的不完全互补型和杂种型。[结论]蛋白质谱和过氧化物酶谱可作为百合杂种鉴定的生化指标及进行目标性状植株的检测。

关 键 词：LiLum davidii var.unicolor Lilium Asiatic hybrids Hybrid F1 PROTEIN PEROXIDASE 

分 类 号：S682.29[农业科学—观赏园艺]