PPARγ、NFκ-B、TNFα-在慢性缺血性肾病大鼠模型中的表达及意义  

Expression and Significance of Peroxisome Proliferator Activated Receptor γ,Nuclear Factor-κB and Tumor Necrosis Factor-α in Rat Model of Chronic Ischemic Nephropathy

作　　者：尹伟英[1] 张莉[2] 冯晓然[1] 申兰雨[1] 徐晗[1] 何艳[1] 肖俊[2] 鄢艳[2] 陈钦开[2] 

机构地区：[1]南昌大学研究生院医学部,2008级南昌330006 [2]南昌大学第一附属医院肾内科,南昌330006

出　　处：《南昌大学学报（医学版）》2011年第1期30-33,37,共5页Journal of Nanchang University：Medical Sciences

摘　　要：目的观察PPARγ、NF-κB、TNFα-在慢性缺血性肾病大鼠模型中的表达,探讨PPARγ、NFκ-B、TNF-α之间的关系及其在慢性缺血性肾病中的作用。方法通过单侧肾动脉部分结扎建立慢性缺血性肾病动物模型。按随机数字表法将16只大鼠分为模型对照组(MC)和假手术组(sham)2组,每组8只。测大鼠术后第4、8、12周动脉血压(SBP)。术后第12周末测24 h尿蛋白、尿β2微球蛋白(β2-MG),然后处死大鼠取肾脏观察肾组织病理改变;分别用免疫组化SP法和RT-PCR法测定肾组织PPARγ、NFκ-B蛋白和PPARγ、NFκ-B、TNF-αmRNA的表达。结果与sham组比较,MC组SBP明显上升,24 h尿蛋白、尿β2-MG水平均明显升高,差异均有统计学意义(均P<0.05)。组织病理检查结果示:与sham组比较,MC组间质有大量炎症细胞侵润,肾小管损伤较明显(均P<0.05)。免疫组化检查结果示:与sham组比较,MC组PPARγ、NF-κB蛋白表达水平均明显升高(均P<0.05)。RT-PCR结果示:与sham组比较,MC组PPARγ、NF-κB、TNFα-mRNA表达水平均升高(均P<0.05)。直线相关性分析表明,MC组大鼠PPARγmRNA表达水平与肾脏病理损害评分及NFκ-B、TNF-αmRNA表达水平均呈正相关(r分别为0.976、0.992、0.954,均P<0.01)。结论慢性缺血使大鼠肾脏PPARγ、NF-κB、TNF-α表达上调,加重肾损害。Objective To observe the expression of PPARγ,NF-κB and TNF-α in rat model of chronic ischemic nephropathy,and to investigate the relationship among PPARγ,NF-κB and TNF-α.Methods Sixteen Sprague-Dawley rats were randomly divided into sham-operated group（sham group,n=8） and model control group（MC group,n=8）.Unilateral renal artery ligation was performed to induce chronic ischemic nephropathy.Arterial blood pressure was measured at weeks 4,8,and 12.Urinary β2-microglobulin and 24-hour urinary protein exretion were measured at the end of 12 weeks,and then pathological changes in renal tissue were observed.The expression of PPARγ,NF-κB and TNF-α was detected by immunohistochemistry and RT-PCR.Results Compared with sham group,the blood pressure,24-hour urinary protein exretion,β2-microglobulin levels,inflammatory cell infiltration,and renal tubular damage significantly increased in MC group（all P0.05）.The levels of protein expression of PPARγ and NF-κB and the levels of mRNA expression of PPARγ,NF-κB and TNF-α markedly increased in MC group compared with sham group（all P0.05）.PPARγ mRNA expression was positively correlated with NF-κB expression,TNF-α expression and renal pathology scores（r=0.976,r=0.992 and r=0.954,respectively,all P0.01）.Conclusion Chronic renal ischemia can increase the expression of PPARγ,NF-κB and TNF-α,and aggravate renal damage.

关 键 词：慢性缺血性肾病 PPARΓ NFΚ-B TNFα- 动物 实验 大鼠 

分 类 号：R-322[医药卫生]