电刺激小脑顶核对缺氧缺血新生大鼠学习记忆能力的影响  

The effect of fastigial nucleus electric stimulation on learning memory ability of rats with hypoxic-ischemic brain damage

作　　者：陶德双[1] 张丽华[2] 谭丽萍[3] 杨本利[2] 李晓红[2] 王立苹[3] 孙颖[3] 

机构地区：[1]佳木斯大学康复医学院,黑龙江佳木斯154002 [2]佳木斯大学康复医学院脑瘫科,黑龙江佳木斯154002 [3]佳木斯大学儿童神经康复实验室,黑龙江佳木斯154002

出　　处：《中国中西医结合儿科学》2011年第2期119-122,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：佳木斯大学重点项目(Sz2010-002)

摘　　要：目的探讨电刺激小脑顶核对缺氧缺血新生大鼠学习记忆功能的影响。方法 7日龄Wist-ar大鼠(36只)随机分为缺氧缺血性脑损伤组和假手术组,缺氧缺血性脑损伤组又分为模型组和电刺激组,采用结扎左侧颈总动脉并吸入氮氧混合气体2h制作新生大鼠缺氧缺血性脑损伤动物模型,分别于手术第2天相同时间点给予无电刺激装置(假手术组),连接装置但不接通(模型组),连接装置并且接通(电刺激组)。各组12只动物,随机分为7d干预组(6只)和14d干预组(6只),两组分别于7、14d干预后,(1)用Y-型迷宫检测脑损伤鼠的学习记忆功能;(2)苏木精-伊红染色法光镜下观察脑神经细胞和神经纤维的病理变化;(3)免疫组织化学检测脑皮质和海马区STAT3蛋白水平的表达。结果 (1)Y-型迷宫测试结果模型组总时间较电刺激组增多,模型组主动回避反应率和正确反应率低于电刺激组(P<0.05);模型组总时间与假手术组相比,显著增加;模型组主动回避反应率和正确反应率也明显低于假手术组,差异均有统计学意义(P<0.05)。(2)假手术组脑组织各部位的结构及细胞层次清楚、形态正常,未见明显损伤性改变。模型组脑组织可见明显的毛细血管出血,细胞核固缩、核碎裂明显,模型建立成功。电刺激组神经细胞变性坏死较少,多数细胞形态相对正常。(3)电刺激组子鼠脑皮质及海马周围信号转导和转录激活蛋白3(STAT3)蛋白较模型组增多(P<0.05)。结论电刺激小脑顶核可促进脑损伤鼠学习记忆能力的恢复。Objective To explore the effect of fastigial nucleus electric stimulation on learning memory of rats with hypoxic-ischemic brain damage.Methods Thirty-six cases of seven-day-age Wistar rats were randomly divided into HIBD group and sham operation group.HIBD group was divided into model group and electric stimulation group.Left common carotid artery was ligatured and nitrogen-oxygen mixture gas inhaled for 2 h,hence HIBD animal models of neonatal rats were made;link the equipment without electrical stimulation（sham operated group）,link the device but not connected（model group）,and link device and connected（electrical stimulation group）at the same time.Twelve rats were randomly divided into 7-day intervention group（6 cases）and 14-day intervention group（6 cases）.After 7 days and 14 days intervention,（1）the two groups were pespectively detected by Y-type maze learning and memory of mice brain injury function;（2）after HE staining,observe nerve cells and nerve fibers by the optical microscope;（3）detect the brain white matter STAT3 protein expression levels by immunohistochemi.Result By Y-type maze test,total time in M group was longer than group E（P 0.05）;active avoid reaction rate and the correct response rate in M group was lower than E group（P 0.05）.In M group the total time increased significantly compared with group F（P 0.05）;in M group,active avoid reaction rate and the correct response rate was also significantly lower than group F（P 0.05）.In F group of structure and cellular level brain tissue of each place was clear,form normal,with no obvious harm change.In M group capillary hemorrhage could be seen obviously,nuclei became pyknotic,nuclear cataclasmproof obvious,so the model as a success.In Group E nerve cells degeneration necrosis was less,most cell forms were relatively normal.The signal transduction and transcription activated protein 3（STAT3）around cortex and seahorses increased relatively more than model group（P 0.05）.Conclusion Electrical stimulati

关 键 词：缺氧缺血性脑病 脑损伤 学习记忆 大鼠 近交系 动物 新生 动物 实验 

分 类 号：R-33[医药卫生]