白花蛇舌草乙醇提取物对人结肠癌细胞HT-29 Pim-1和Pim-2 mRNA表达的影响  

Effect of Herba Hedyotis Diffusa Ethanol Extract on mRNA Expression of Pim-1 and Pim-2 in Human Colon Cancer HT-29 Cells

作　　者：魏丽慧[1,2] 林久茂[1,2] 彭军[1,2] 徐伟[1] 庄群川[1,2] 赵锦燕[1] 

机构地区：[1]福建中医药大学,福建福州350108 [2]福建中西医结合研究院,福建福州350108

出　　处：《世界中西医结合杂志》2011年第4期284-287,共4页World Journal of Integrated Traditional and Western Medicine

基　　金：福建省自然科学基金项目(No.2010J01195);福建省中西医结合老年性疾病重点实验室(福建中医药大学)开放课题(No.2008J1004);福建中医药大学科研基金项目(No.X2009063)

摘　　要：目的探讨白花蛇舌草乙醇提取物对人结肠癌细胞HT-29 Pim-1和Pim-2 mRNA表达的影响。方法采用人结肠癌细胞HT-29常规体外培养,随机设定空白组和不同浓度(1、3、5、10 mg.mL-1)白花蛇舌草乙醇提取物组,干预24 h。倒置显微镜观察细胞形态变化;MTT法测定不同浓度药物对HT-29细胞增殖的影响,并用5 mg.mL-1浓度干预1、3、6、12、24、48 h观察对HT-29细胞增殖的影响;RT-PCR检测药物作用后HT-29细胞Pim-1和Pim-2 mRNA的表达。结果白花蛇舌草乙醇提取物干预后,HT-29的细胞密度明显减少,细胞变小,最终悬浮脱落而死亡;HT-29细胞增殖受到抑制,并呈现出量效和时效关系;白花蛇舌草乙醇提取物能明显下调HT-29细胞Pim-1和Pim-2 mRNA表达,并随药物浓度的增加而减少,呈一定的量效作用。结论白花蛇舌草乙醇提取物能显著抑制人结肠癌HT-29细胞的增殖以及下调Pim-1和Pim-2的mRNA表达。Objective To investigate the impacts of herba hedyotis diffusa ethanol extract on mRNA expression of Pim-1 and Pim-2 in human colon carcinoma HT-29 cells.Methods Conventional in vitro culture was adopted for human colon carcinoma HT-29 cells.Blank group and herba hedyotis diffusa ethanol extract groups of different concentrations（1,3,5,10 mg·mL-1）were divided and had received intervention for 24 h.The reversed microscope was used to observe the changes in cellular morphology.MTT assay was adopted to determine the impacts on HT-29 cell proliferation with the medicine of different concentrations.Moreover,the medicine 5 mg·mL-1 was used in the intervention for 1,3,6,12,24 and 48 h in succession and its impacts on HT-29 cell proliferation was observed.RT-PCR assay was used to determine mRNA expression of Pim-1 and Pim-2 afterwards.Results After the intervention with herba hedyotis diffusa ethanol extract,HT-29 cell density reduced apparently,the cells became smaller and were suspended,fallen and dead finally.HT-29 cell proliferation was inhibited and dose-effect and time-effect relationships presented.Herba hedyotis diffusa ethanol extract down-regulated apparently mRNA expression of HT-29 cell Pim-1 ad Pim-2 and the expression decreased while the increasing of medicine concentration,indicating a certain dose-time relationship.Conclusion Herba hedyotis diffusa ethanol extract inhibits significantly HT-29 cell proliferation and down-regulates mRNA expression of Pim-1 and Pim-2 in human colon carcinoma.

关 键 词：白花蛇舌草 结肠癌 PIM-1 PIM-2 

分 类 号：R735.35[医药卫生—肿瘤]