麻黄多糖中蛋白含量测定及脱蛋白方法的比较  

Determination of Contents of Protein in Polysaccarides from the Stems of Ephedra Sinica Stapf and Comparison of Different Methods for Deproteinization

作　　者：梁军[1] 夏永刚[1] 杨炳友[1] 王秋红[1] 匡海学[1] 

机构地区：[1]黑龙江中医药大学北药基础与应用研究省部共建教育部重点实验室黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040

出　　处：《中医药学报》2011年第2期73-75,共3页Acta Chinese Medicine and Pharmacology

基　　金：十二五国家科技部重大专项课题"重大新药创制"专项课题(编号:2011ZX09102-006-01);国家自然科学基金面上项目(编号:30973870);黑龙江中医药大学博士创新基金(编号:B201002);黑龙江省博士后科研基金(2010)

摘　　要：目的:建立麻黄多糖中蛋白的含量测定方法,比较不同脱蛋白方法的蛋白脱除效果。方法:水提醇沉提取麻黄多糖,Sevag法、三氯乙酸法、木瓜蛋白酶-Sevag法去除蛋白,考马斯亮蓝法测定蛋白质含量。结果:麻黄多糖脱蛋白前的蛋白含量为26.30%,Sevag法、三氯乙酸法和木瓜蛋白酶-Sevag法脱蛋白后的蛋白含量分别为15.00%,6.80%和1.70%;三种方法的蛋白脱除率分别为42.97%,74.14%和93.54%。结论:木瓜蛋白酶-Sevag法的脱蛋白效果较好,考马斯亮蓝G-250染色法操作简便,准确性好,不失为多糖中蛋白含量测定的一种好方法。Objective： To determine the protein content and comparison of different methods of removing protein in polysaccarides from the stems of Ephedra sinica Stapf.Methods： The polysaccharide was extracted with the method of water-extraction and alcohol precipitation,the protein was dispelled with the Sevag,TCA and Enzyme-Sevag method,respectively.The content of protein was measured with method of Coomassie Brilliant Blue.Results： The contents of protein in Mahuang polysaccharide were 26.30% before deproteinization,and the contents of protein after deproteinization with methods Sevag,TCA,Enzyme-Sevag were 15.00%,6.80% and 1.70%,respectively.The percentage of removed protein for the three methods were 42.97%,74.14% and 93.54%,respectively.Conclusion： It is concluded that the enzyme-Sevag removal of protein is an effective method.The coomassie breiliant blue G-250 method is simple and accurate.It provides an excellent method for the determination of protein in polysaccarides.

关 键 词：麻黄多糖 蛋白含量测定法 脱蛋白 

分 类 号：R284.1[医药卫生—中药学]