5-氮杂-2′-脱氧胞苷对胃癌AGS细胞CHFR基因去甲基化作用及意义  

Demethylation of CHFR gene in gastric cancer cell line AGS by 5-Aza-CdR and its clinical applications

作　　者：邱庆安[1] 吕云福[1] 陈一明[1] 林友刚[1] 伍海鹰[1] 刘宁[1] 

机构地区：[1]海南省人民医院,海口570311

出　　处：《山东医药》2011年第14期6-7,共2页Shandong Medical Journal

摘　　要：目的观察5-氮杂-2′-脱氧胞苷(5-Aza-CdR)对胃癌AGS细胞CHFR基因去甲基化的作用,并探讨其临床意义。方法分别采用BSP和RT-PCR技术检测5-Aza-CdR处理前后胃癌AGS细胞CHFR基因启动子甲基化状态及其mRNA。结果 AGS细胞CHFR基因启动子在5-Aza-CdR处理前呈现高甲基化状态(甲基化率≥60%),其mRNA表达完全缺失;5-Aza-CdR处理后则表现为低或无甲基化状态(甲基化率≤20%),其mRNA表达恢复正常。结论 CHFR基因启动子在AGS细胞中呈高甲基化状态,5-Aza-CdR能显著逆转其CHFR基因异常甲基化,诱导CHFR基因表达,为胃癌的治疗提供新思路。Objective To explore demethylation effect of 5-Aza-2′deoxycytidine（5-Aza-CdR）in gastric cancer cell line AGS and study its clinical applications.Methods Bisulfite sequencing PCR（BSP） and reverse transcriptase PCR（RT-PCR） were used to detect CHFR gene promoter′s methylation status and its mRNA expression pre and post 5-Aza-CdR treatment in gastric cancer cell line AGS respectively.Results CHFR gene promoter displayed hypermethylated in gastric cancer cell line AGS（methylation rate≥60%）,while it was low after 5-Aza-CdR treatment（methylation rate≤20%）;CHFR mRNA expression recovered after 5-Aza-CdR′s demethylation.Conclusions CHFR gene′s promoter display hypermethylation status in gastric cancer cell line AGS,5-Aza-CdR can recovery abnormal hypermethylation and induce its mRNA expression significantly.It may be a new idea for the treatment of gastric cancer.

关 键 词：5-氮杂-2′-脱氧胞苷 CHFR基因 去甲基化 胃癌 胃癌细胞 

分 类 号：R735.35[医药卫生—肿瘤]