牛蒡子苷元对肝癌SMMC-7721细胞增殖、凋亡的影响及机制探讨  

Effect and mechanism of proliferation and apoptosis induced by arctigenin in human hepatocellular carcinoma SMMC-7721 cell

作　　者：郑国灿[1] 王兵[1] 钱程佳[1] 

机构地区：[1]东南大学附属中大医院,南京210009

出　　处：《山东医药》2011年第14期13-15,共3页Shandong Medical Journal

基　　金：东南大学国家自然科学基金预研基金项目(XJ0650261)

摘　　要：目的观察牛蒡子苷元(ARG)对肝癌SMMC-7721细胞增殖、凋亡的影响,并探讨其机制。方法将不同浓度的ARG作用于SMMC-7721细胞,并设不加ARG对照组。MTT法测算细胞增殖抑制率、流式细胞术检测细胞周期及凋亡率,RT-PCR法检测细胞中的Bcl-2 mRNA。结果与对照组比较,随ARG浓度增加,SMMC-7721细胞增殖率逐渐降低(P均<0.05),G0/G1期细胞比例显著增加(P均<0.05),G2、M、S期细胞比例减少(P均<0.05);随ARG浓度增加、作用时间延长,SMMC-7721细胞凋亡率显著增加(P均<0.05),Bcl-2 mRNA表达量减少(P均<0.05)。结论 ARG可明显抑制SMMC-7721细胞增殖并诱导其凋亡;可能与ARG下调细胞中Bcl-2基因的表达有关。Objective To evaluate the effect of proliferation and apoptosis induced by arctigenin（ARG） in human hepatocellular carcinoma SMMC-7721 cell,and explore its mechanism.Methods SMMC-7721 cell were treated with different concentration ARG.Proliferation and apoptosis of SMMC-7721 cell inhibited by ARG were assessed by MTT and flow cytometry（FCM）.Expression levels of Bcl-2 mRNA in SMMC-7721 cell were determined by RT-PCR.Results The proliferation of SMMC-7721 cells were significantly lower in ARG Group than in the control group（P0.05）.FCM showed the ratio of G0/G1 increased（P0.05）.RT-PCR showed that the expression of Bcl-2 reduced（P0.05）.Conclusion ARG can inhibit the proliferation,block the cell cycle at G0/G1 and promotes apoptosis of SMMC-7721 cells,and the mechanism of apeptosis is dependent on the activity of Bcl-2.

关 键 词：牛蒡子苷元 肝肿瘤 原发性肝癌 细胞凋亡 BCL-2基因 

分 类 号：R735.7[医药卫生—肿瘤]