木醋杆菌的培养基优化研究  

Medium Optimization of Acetobacter xylinum HN001

作　　者：杜晶晶[1,2] 曹献英[1,2] 林强[1,2] 陈胜杰[1,2] 胡凌俊[1,2] 

机构地区：[1]海南省优势资源化工材料应用技术教育部重点实验室 [2]海南大学材料与化工学院,海南海口570228

出　　处：《纤维素科学与技术》2011年第1期35-40,共6页Journal of Cellulose Science and Technology

基　　金：国家自然科学基金项目(50763002);海南省自然科学基金项目(509001);海南大学科研项目(hd09xm30)

摘　　要：采用六因素三水平正交实验对木醋杆菌Acetobacter xylinum HN001的培养基进行了优化,确定了最佳培养基组成为:葡萄糖30 g/L,蛋白胨7.5 g/L,酵母粉10 g/L,磷酸氢二钠7.5 g/L,柠檬酸0.5 g/L,pH 5.0,30℃。采用优化的培养基制备细菌纤维素,应用傅立叶红外光谱、热重分析和扫描电子显微镜对其结构和性能进行表征。红外光谱测试显示含有纤维素的特征吸收峰。热重分析结果表明细菌纤维素最大失重温度为319.06℃,热稳定性较好。扫描电镜观察到细菌纤维素的纳米网状结构。The medium optimization of Acetobacter xylinun HN001 was obtained by orthogonal experimental design.The culture media of Acetobacter xylinun HN001 was optimized as：Glucose 30 g/L,peptone 7.5 g/L,yeast extract 10 g/L,Na2HPO4 7.5 g/L,Citric acid 0.5 g/L,pH 5.0,30℃.In this study,bacterial cellulose was synthesized by incubation of Acetobacter xylinun HN001 in the modified culture medium.And they were characterized by FT-IR spectrum,scanning electron microscope（SEM） and thermo gravimetric analyzer.The characteristic adsorption peaks of bacterial cellulose（BC） were in FTIR spectra.The maximum weight loss temperature of BC was 319.06℃,thermal stability of BC was better.Nanostructure of BC was observed by SEM.

关 键 词：木醋杆菌 培养基优化 

分 类 号：Q936[生物学—微生物学]