依达拉奉对大鼠癫痫持续状态后脑自由基代谢及海马神经元凋亡的影响  

Effects of edaravone on free radical metabolism and hippocampal neuron apoptosis in rats following status epilepticus

作　　者：薛国芳[1] 李光来[1] 赵宝珍[2] 李东芳[1] 牛海雁 郑辑英[1] 王改青[1] 陈文军[1] 齐登斌[1] 

机构地区：[1]山西医科大学第二医院神经内科,太原030001 [2]山西医科大学第二医院内分泌科,太原030001 [3]山西省长治市人民医院神经内科

出　　处：《中国药物与临床》2011年第4期385-387,共3页Chinese Remedies & Clinics

基　　金：山西医科大学第二医院青年基金(20090202)

摘　　要：目的观察依达拉奉对大鼠癫痫持续状态后脑内自由基及海马神经元凋亡的影响及可能机制。方法采用氯化锂-匹罗卡品大鼠癫痫持续状态模型,成年雄性Wistar大鼠54只随机分为实验组48只和对照组6只,实验组分为模型组、依达拉奉治疗组,每组按照时间点分为4个亚组(12、24、72 h,7 d)。化学比色法测定脑组织匀浆中超氧化物歧化酶(SOD)活性、丙二醛(MDA)的含量,免疫组织化学法检测大鼠脑组织c-fos蛋白表达。结果与对照组相比,模型组的SOD活性在12 h开始下降,72 h时最低(P<0.01),7 d时恢复正常;依达拉奉治疗组与之趋势相同,但各时间点均高于模型组。模型组的MDA含量和大鼠脑组织的c-fos蛋白表达在各时间点均高于对照组(P<0.05);依达拉奉治疗组与之趋势相同,但在24、72 h低于模型组(P<0.05)。结论依达拉奉可以清除自由基,减少海马神经元的凋亡,对癫痫持续状态后的脑损伤有一定的保护作用。Objective To investigate the effects of edaravone on free radical metabolism and hippocampal neuron apoptosis in rats following status epilepticus（SE）,and the possible mechanism that underlies such effects.Methods Lithium-pilocarpine induced SE models were recruited in this study.A total of 54 male Wistar rats were randomly divided into the normal control group（n=6） and the experimental group（n=48）.The experimental group included SE models（M group） and edaravone treated rats（ED group）,and the both were further divided into 4 subgroups by time spots（12 h,24 h,72 h and 7 d）.The activity of superoxide dismutase（SOD） and contents of malondialdehyde（MDA） in the brain homogenate were measured by chemical colorimetric analysis,and c-fos protein expression in rat brain tissues detected by immunohistochemistry.Results Compared with the normal control group,the M group started to show reduced SOD activity at 12 h,which reached a nadir at 72 h（P0.01） and returned to normal at 7 d.The ED group had a similar trend as in the M group but much higher SOD activity at different time spots.Both the contents of MDA and expression level of c-fos protein in M group were significantly higher than those in the control group at different time spots（P0.05）.The ED group had a similar trend as in the M group but much lower contents of MDA and expression level of c-fos protein at 24 h and 72 h（P0.05）.Conclusion Edarovne may scavenge the free radicals and ameliorate hippocampal neuron apoptosis,which may have certain protective effects against the brain injury after SE.

关 键 词：癫痫持续状态 自由基 C-FOS蛋白 

分 类 号：R742.1[医药卫生—神经病学与精神病学]