基质金属蛋白酶-2血清学和尿脱落细胞荧光原位杂交技术联合检测在膀胱癌的应用  

Significance of FISH and serum MMP-2 detecting in the bladder urothelial carcinoma

作　　者：韩永[1] 徐燕杰[1] 黄海燕[1] 许晓光[1] 冯凯[1] 蔡明[1] 石炳毅[1] 郭晖[2] 

机构地区：[1]中国人民解放军第309医院,100091 [2]华中科技大学同济医院

出　　处：《山西医药杂志（上半月）》2011年第4期323-325,共3页Shanxi Medical Journal

基　　金：国家科技支撑计划(2008BAI60B04);卫生部科研基金(WKJ2007-3-001)

摘　　要：目的探讨基质金属蛋白酶(MMP)-2、尿脱落细胞荧光原位杂交联合检测对膀胱肿瘤诊断的价值。方法 100例膀胱肿瘤患者的MMP-2水平用酶联免疫吸附试验(ELISA)检测含量,荧光原位杂交技术尿脱落细胞表达,20名健康人群标本对照。结果膀胱癌患者MMP-2水平,荧光原位杂交阳性显著高于健康对照组(P<0.05)。联合检测可明显提高膀胱癌阳性检出率,降低患者费用。结论 MMP-2水平与尿脱落细胞荧光原位杂交联合检测对膀胱癌的诊断有重要参考价值,弥补了单独荧光原位杂交检测的不足。Objective To investigate the value of serum metalloptoteinase-2（MMP-2） and fluorescence in situ hybridization（FISH） in diagnosis of the bladder urothelial carcinoma.Methods Twenty cases of healthy adults and 100 cases of patients with bladder cancer were involved.The nucleus of shedding cells were detected by FISH and serum MMP-2 contents were detected in 100 patients with transitional cell carcinoma of bladder and 20 healthy controls by enzyme-linked immunosorbent assay（ELISA）.Results The levels of both MMP-2 and FISH in cases of malignant the bladder urothelial tumors were significantly higher as compared with those of the benign and controls（P0.05）.The positive staining had no relevance to age,gender,and the type of tumor（P0.05）.Conclusion Combined MMP-2 and FISH detection is of important value in the diagnosis of the bladder urothelial tumors and may be an additional parameter for FISH in differential diagnosis.

关 键 词：膀胱肿瘤 明胶酶A 原位杂交 荧光 

分 类 号：R737.14[医药卫生—肿瘤]