重组人促红细胞生成素体外诱导人脐静脉内皮细胞血管新生作用的实验研究  

Angiogenic Role of Recombinant Human Erythropoietin on EA.hy926 Cell Line:an in vitro Study

作　　者：姚晓艳[1] 李红戈[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022

出　　处：《神经损伤与功能重建》2011年第2期79-83,共5页Neural Injury and Functional Reconstruction

摘　　要：目的:探讨重组人促红细胞生成素(rH-EPO)对缺血缺氧损伤内皮细胞的血管新生作用及其可能机制。方法:氧糖剥夺-复氧复糖(OGD/R)损伤内皮细胞株EA.hy926,复氧复糖过程中添加rH-EPO;采用MTT还原法、Transwell小室迁移实验及Matrigel小管形成实验评价内皮细胞血管新生能力;ELISA法检测培养上清液血管内皮生长因子(VEGF)含量;实时PCR法分析内皮型一氧化氮合酶(eNOS)mRNA变化;Western Blot法分析eNOS蛋白变化。结果:OGD诱导EA.hy926细胞受损,降低其增殖、迁移和管样结构形成能力,VEGF含量减少,eNOS基因及其蛋白表达均下降;rH-EPO干预后,受损细胞的增殖、迁移和管样结构形成能力提高,VEGF含量、eNOS基因及其蛋白表达均增加(均P<0.05)。结论:rH-EPO可促进受损内皮细胞血管新生,VEGF和eNOS可能参与rH-EPO诱导的血管新生过程。Objective： To study the angiogenic role of recombinant human erythropoietin（rH-EPO） on EA.hy926 cells after oxygen and glucose deprivation followed by reoxygenation（OGD/R） in vitro.Methods： Cells were subjected to OGD/R and stimulated by rH-EPO during the reoxygenation procedure.MTT assay,Transwell assay and Matrigel assay were used to detect the angiogenic ability of cells post-OGD.The expressions of vascular endothelial growth factor（VEGF）,endothelial nitric oxide synthase（eNOS） mRNA and eNOS protein were also detected.Results： OGD induced a significant decrease in proliferation,migration,and tube formation ability of EA.hy926 cells and the expression of VEGF,eNOS mRNA and protein were significantly decreased.rH-EPO mitigated the OGD-induced damage of proliferation,migration,and tube formation ability of cells and increased the expression of VEGF,eNOS mRNA and protein.Conclusion： VEGF and eNOS probably play critical roles in the angiogenic effects of rH-EPO on cells after OGD.

关 键 词：重组人促红细胞生成素 血管新生 氧糖剥夺 血管内皮生长因子 内皮型一氧化氮合酶 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.05[医药卫生—临床医学]