聚乙二醇化降纤酶对血小板聚集和凝血功能的影响  

Effects of PEGylated defibrase on platelet aggregation and coagulability activity

作　　者：郭月芳[1] 谢炜[1] 王玉梅[1] 吉蕊[1] 盛雨辰[1] 

机构地区：[1]上海医药工业研究院药理研究室,上海200437

出　　处：《中国药理学通报》2011年第4期512-515,共4页Chinese Pharmacological Bulletin

基　　金：国家"重大新药创制"科技重大专项资助项目(No2009ZX09301-007)

摘　　要：目的研究聚乙二醇化降纤酶对血小板聚集和凝血功能的影响。方法以二磷酸腺苷(ADP)诱导血小板聚集,用Labor aggregometer-153型双道血小板聚集仪测定血小板最大聚集率,观察聚乙二醇化降纤酶对大鼠体内血小板聚集的影响;同时通过对Beagle犬各个时间点凝血指标:凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)和纤维蛋白原(FIB)含量的观察,研究聚乙二醇化降纤酶对beagle犬凝血功能的影响。结果聚乙二醇化降纤酶低、中、高剂量组均能明显抑制ADP诱导的大鼠血小板聚集作用;降低纤维蛋白原(FIB)含量,延长beagle犬TT、PT、APTT时间,等剂量的聚乙二醇化降纤酶药效达峰和维持时间均较阳性药降纤酶的长。结论聚乙二醇化降纤酶具有较强的对抗血小板聚集和凝血作用。Aim To investigate the effects of PEGylated defibrase on platelet aggregation and coagulation function.Methods The platelet aggregation rate（PAGT） induced by adenosine diphosphate（ADP） was assayed by labor aggregometer-153 platelet aggregation analyzer to study the effects of PEGylated defibase on platelet aggregation in rats.Thrombin time（TT）,protrombin time（PT）,activated partial thromboplasting time（APTT） and fibrinogen（FIB） were observed at the same time to study the effects of PEGylated defibrase on coagulation function in beagles.Results All doses of PEGylated defibrase significantly inhibited platelet aggregation in rats induced by ADP,and prolonged TT,PT,and APTT in beagles.FIB was also decreased after the treatment by PEGylated defibrase,while the peak time and the lasting time in PEGylated defibrase group were longer than those in the positive group.Conclusion PEGylated defibrase exerts a remarkable effect on antiplatelet aggregation and coagulation function.

关 键 词：降纤酶 聚乙二醇 凝血酶时间 凝血酶原时间 活化部分凝血活酶时间 纤维蛋白原 血小板聚集 

分 类 号：R-332[医药卫生] R331.124