心梗所致心衰大鼠模型复制的改良初探及评价  

The improvement and evaluation of the myocardial infarction-induced heart failure model in rats

作　　者：喻斌[1,2] 周静[2] 吕高红[2] 许惠琴[2] 李伟 方泰惠[1] 

机构地区：[1]南京中医药大学药学院江苏省中药药效与安全性评价重点实验室,江苏南京210046 [2]南京中医药大学药学院中药药理学省部共建重点实验室,江苏南京210046 [3]南京中医药大学药学院药学系,江苏南京210046

出　　处：《中国药理学通报》2011年第4期577-580,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No81072542);国家科技重大专项资助项目(No2009ZX09103-081);江苏省中药药效与安全性评价重点实验室开放课题(NoP09010)

摘　　要：目的对心梗所致心衰大鼠模型的复制提供改良方法,并提供相应的指标评价体系。方法在常规大鼠心梗诱导心衰模型的基础上,对大鼠体重、麻醉药剂量、气管插管方式、心脏暴露方法、缝合处理、心肺按压处理等进行优选和改良。造模后进行死亡率、体征、超声心动图、血流动力学、左心室微循环、左室质量指数、心肌病理学和超微结构检查。结果与常规方法相比,本实验心衰组大鼠术后死亡率可见减少。同时射血分数(EF)、左室短轴缩短率(LVFS)、心输出量(CO)、每搏输出量(SV)、心脏指数(CI)明显减少,左室收缩末期容积(LVESV)、左室质量(LVW)、心脏质量(HM)、左室质量指数(LVMI)、心脏质量指数(HMI)明显增加,左心室微循环灌注不足。而组织病理学检查发现心衰组心肌细胞肥大,肌纤维排列紊乱,大量纤维组织增生,炎细胞浸润。超微结构中也发现线粒体、核膜以及肌纤维均发生不同程度的损伤。结论本实验中的模型复制方法能明显提高心衰大鼠存活率和模型复制成功率,并为心衰模型提供较为全面的评价体系。Aim To provide a improved method of establishing myocardial infarction-induced heart failure model in rats and a comprehensive evaluation system.Method On the basis of the conventional means of establishing heart failure rats model derived from myocardial infarction,we optimized the procedures by modifying the choice of weight,dose of anaesthetics,management of intubation,exposure of heart,treatment of suture and cardiopulmonary press.After coronary ligature,we observed the death rate,physical signs,echocardiography,hemodynamic,left ventricular microcirculation,mass index,pathology and ultrastructure of failure rats.Results The way of constructing failure model of rats in this paper could decrease death rate compared with the conventional method.At the same time,EF,LVFS,CO,SV and CI decreased obviously compared with the sham group,LVESV,LVW,HM,LVMI and HMI increased significantly comparing with the sham group.We also discovered the disturbance of left ventricular microcirculation and abnormality of histology and ultrastructure in failure rats.Conclusion The method of creating heart failure rats in this paper could increase the survival rate and success rate of the model.In addition,a better evaluation system of cardiac failure was also established in this study.

关 键 词：心梗所致心衰 模型改良 超声心动图 血流动力学 超微结构 评价体系 

分 类 号：R-332[医药卫生] R322.11