液相芯片技术在人乳头瘤病毒分型检测中的应用  

Genotyping of human papillomavirus by Luminex method

作　　者：彭春梅[1] 邓先连 林斌 胡守旺 陈晓光[1] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515 [2]福建省分子诊断技术工程实验室,福建福州350002 [3]广州华银医学检验中心,广东广州510665

出　　处：《热带医学杂志》2011年第3期249-252,共4页Journal of Tropical Medicine

基　　金：国家"863"项目(2006AA020902)

摘　　要：目的构建人乳头瘤病毒(HPV)基因分型的液相芯片,针对中国常见的23种型别的HPV进行分型检测,并探讨其在临床应用中的特异性和敏感性。方法采用HPV通用型引物MY09/11以及一条简并引物对临床样本进行PCR扩增;针对HPV不同型别设计分型探针,并偶联到Luminex微球上,通过LuminexTM平台对PCR产物进行分型检测。同时以测序法作为验证标准,对液相芯片法(Luminex法)检测结果进行评价。结果以测序法作为验证标准,Luminex法对314例HPV样本的分型检测结果为灵敏度98.10%,特异性98.08%,符合率98.09%。Kappa一致性检验结果为0.962。结论 Luminex法用于HPV感染样本中的分型检测具有高灵敏度和特异性,可以用于临床HPV诊断。Objective To establish a specific and sensitive Luminex method for the detection of human 23 subtypes of papillomavirus（HPV） subtypes.Methods HPV DNA was amplified from clinical specimens by polymerase chain reaction with a pair of universal primers MY09/11 and a degenerate primer,and then genotyped with liquid chip hybridization method（Luminex method）.Simultaneously,the samples were detected by sequencing method which was taken as the gold standard.Sequencing method was used as the standard method to check for the genotype.Results The sensitivity and specificity of the Luminex method was 98.10% and 98.08%,respectively.Kappa 0.962.Conclusion The Luminex method is sensitive and specific for the detection the HPV subtypes.This is a high-throughput method with an advantage of convenience in clinical diagnosis.

关 键 词：液相芯片 人乳头瘤病毒 分型检测 测序 

分 类 号：R737.33[医药卫生—肿瘤]