原发性胆汁性肝硬化患者外周血单个核细胞TRAF2表达上调及其临床意义  

Increased expression of tumor necrosis factor associated receptor factor 2 in the peripheral blood mononuclear cells of patients with primary biliary cirrhosis and its clinical significance

作　　者：孙懿[1] 韩志君[2] 胡志德[1] 周晔[1] 谷明莉[1] 姚定康[3] 邓安梅[1] 仲人前[1] 

机构地区：[1]第二军医大学长征医院实验诊断科,全军医学免疫诊断中心,全军临床免疫重点实验室,上海200003 [2]无锡市第二人民医院检验科,无锡214002 [3]第二军医大学长征医院消化科,上海200003

出　　处：《现代免疫学》2011年第2期117-120,共4页Current Immunology

基　　金：国家自然科学基金资助项目(30770997、30772017、30972730);上海市科委基金资助项目(09JC1405400、08QH14001)

摘　　要：研究肿瘤坏死因子受体相关因子(TRAF)在原发性胆汁性肝硬化(PBC)患者外周血单个核细胞(PBMC)中的表达及其与疾病发生发展的关系。分别采用实时荧光定量PCR和免疫印迹法,检测47例PBC和47例健康对照者中TRAF1和TRAF2的基因和蛋白表达。结果表明:PBC患者PBMC中TRAF2 mRNA和蛋白的表达明显高于健康对照组(P<0.05),而TRAF1在两者中表达无明显区别。PBC患者Ⅲ、Ⅳ期PBMC中的TRAF2的mRNA和蛋白的表达较Ⅰ、Ⅱ期明显增高(P<0.05);提示:TRAF2的表达与PBC的发生发展存在一定的相关性,在PBC的发病机制起到重要的作用,为PBC的诊断和预防提供了新线索。To investigate the role of tumor necrosis factor receptor-associated receptor factor（TRAF） in the pathogenesis of primary biliary cirrhosis（PBC）,peripheral blood mononuclear cell（PBMC） was isolated from 47 PBC patients and 47 healthy controls,the relative expression of TRAF1 and TRAF2 were detected by real-time reverse transcription polymerase chain reaction and Western-blotting at mRNA and protein level respectively.The results showed that the expression of TRAF2,but not TRAF1,was increased in PBC patients.In addition,the expression of TRAF2 was significantly higher in patients in stage Ⅲ and Ⅳ of compared with stage Ⅰ and Ⅱ of diseases.Our work indicates that TRAF2,but not TRAF1 is involved in the pathogenesis of PBC.Thus,TRAF2 may be a potential therapy or prevention target involved in the regulatory mechanisms in the development of PBC.

关 键 词：原发性胆汁性肝硬化 肿瘤坏死因子受体相关因子 实时荧光定量PCR 免疫印迹法 

分 类 号：R575.2[医药卫生—消化系统]