江苏汉族群体D12S391和D6S1043基因座遗传多态性研究  

Genetic polymorphisms of D12S391 and D6S1043 in Han ethnic group of Jiangsu Province

作　　者：刘德丽[1,2] 周旭科[1,3] 姜玉婷[1] 高玉振[1] 

机构地区：[1]苏州大学医学部法医学系,苏州215123 [2]江苏省南通市公安局刑警支队 [3]浙江省诸暨市公安局刑侦大队

出　　处：《南通大学学报（医学版）》2011年第2期87-88,91,共3页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(30800621)

摘　　要：目的:调查D12S391和D6S1043基因座在江苏汉族人群的群体遗传学数据,为亲子鉴定和个人识别工作提供数据基础。方法:利用AmpF1STR Sinofiler试剂盒进行多重PCR扩增,3130型遗传分析仪进行毛细管电泳,Gen-eMapper软件自动分析各基因座的基因型。PowerStats软件计算两个基因座的等位基因频率、杂合度、个人识别机率、非父排除率及多态信息含量等群体遗传学参数,χ2检验验证Hardy-Weinberg平衡。结果:D12S391和D6S1043基因座在江苏汉族群体中均有较高的多态性,其非父排除概率和个人识别率分别为0.616、0.638、0.946和0.964。两个基因座的基因型分布均符合Hardy-Weinberg平衡(P>0.05)。结论:本研究获得了D12S391和D6S1043两个基因座在江苏汉族群体的群体遗传学数据,分析结果显示这两个基因座适合江苏汉族群体法医学应用。Objective：To investigate the population genetic data of D12S391 and D6S1043 loci in Han ethnic group of Jiangsu Province and provide database reference for personal indentification and paternity testing.Methods： Genotyping were performed using AmpF1STR Sinofiler kit and capillary electrophoresis.PowerStats software was used to calculate population genetic parameters.Deviation from Hardy-Weinberg equilibrium was tested using chi-square test.Results： Allelic frequencies indicated D12S391 and D6S1043 loci were highly polymorphic in Han ethnic group of Jiangsu Province.The exclusion power and discrimination power were 0.616,0.638,0.946 and 0.964 respectively.Conclusion： Population genetic data were obtained for D12S391 and D6S1043 loci in Han ethnic group of Jiangsu Province.Statistic results showed that these loci were suitable for forensic applications in our region.

关 键 词：法医遗传学 短串联重复序列 D12S391 D6S1043 汉族 江苏省 

分 类 号：Q755[生物学—分子生物学]