小鼠大肠癌肝转移微创模型的建立与评价  

Establishment and evaluation of hepatic metastasis model of colorectal carcinoma in mice

作　　者：董亮[1] 孙茂民[2] 匡玉庭[3] 王守立[1] 王影影[1] 纪辉[3] 柴玉海[1] 

机构地区：[1]苏州大学医学部病理系,苏州215123 [2]苏州大学医学部解剖学与组织胚胎学系,苏州215123 [3]苏州大学医学部附属第一医院普外科

出　　处：《南通大学学报（医学版）》2011年第2期89-91,2,共3页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(81072186);江苏省高校自然科学基金资助项目(10KJB320018)

摘　　要：目的:根据大肠癌肝转移模型的手术损伤程度筛选合适的建模方法。方法:以BALB/c小鼠为对象,随机分为3组,分别经直肠黏膜内、腹腔外脾内和腹腔内脾原位等方法注射0.2 mL鼠结肠癌细胞株CT26(浓度106个/mL),观察小鼠成瘤情况、肝转移率,并用酶联免疫吸附实验检测各组小鼠建模后5 d INF-γ的表达水平。结果:3种方法均能建立大肠癌肝转移模型,直肠黏膜内、腹腔外脾内和腹腔内脾原位注射法的肝转移率分别为37.5%、87.5%和87.5%;各组建模7 d后血清中INF-γ的水平分别为345.8、1 085.2和680.4 pg/mL,腹腔外脾内注射法INF-γ的表达水平较其它2组明显增高(P<0.05)。结论:脾内原位注射法是一种建立大肠癌肝转移最有效且损伤最小的一种方法,比较适合用来研究大肠癌肝转移的相关分子机制。Objective： To select optimal method to establish hepatic metastasis models of colorectal carcinoma（CRC） based on degree of transplanting damage for studying mechanism of hepatic metastasis of CRC.Methods： BALB/c mice were randomly divided into 3 groups with injection of 0.2 mL CT26 CRC cells（1×106/mL）by rectum transplanting,out of peritoneal cavity spleen transplanting and intraperitoneal spleen transplanting respectively.The hepatic metastasis was observed,and INF-γ was detected by enzyme linked immunosorbent assay（ELISA） after 5 d.Results： All of the three groups inoculated with CT26 cells developed hepatic metastasis.The hepatic metastasis rates were 37.5%,87.5% and 87.5% respectively in rectum transplanting group,out of peritoneal cavity spleen transplanting group and intraperitoneal spleen transplanting group.Levels of INF-γ in blood were 345.8,1 085.2 and 680.4 pg/mL respectively,and INF-γ in intraperitoneal spleen transplanting group was increased,compared with that in rectum transplanting group and out of peritoneal cavity spleen transplanting group（P0.05）.Conclusion： The intraperitoneal spleen transplanting is the most effective and the least injury way for establishing hepatic metastasis models of CRC and is a suitable method for studying mechanism of hepatic metastasis of CRC.

关 键 词：直结肠癌 肝转移 模型 微创 小鼠 

分 类 号：R735.37[医药卫生—肿瘤]