NF-kB抑制剂PDTC对人骨肉瘤MG-63细胞凋亡及Livin和Caspase-9表达的影响  

Effects of PDTC on apoptosis and expression of livin and caspase-9 on human osteosarcoma cell MG-63

作　　者：王玉峰[1] 王岩峰[1] 胡春吉[1] 柴利鹏[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001

出　　处：《中国矫形外科杂志》2011年第7期589-591,共3页Orthopedic Journal of China

基　　金：辽宁省教育厅科技研究项目(编号:2008557)

摘　　要：[目的]观察NF-kB抑制剂PDTC对人骨肉瘤MG-63细胞凋亡及Livin和Caspase-9表达的影响。[方法]体外培养人骨肉瘤MG-63细胞系,用50、100、200和400μmol/L的PDTC作用于骨肉瘤MG-63细胞24、48、72 h后,用MTT法测各组骨肉瘤MG-63细胞存活率,用流式细胞仪测细胞的凋亡率。用Western Blot方法检测不同浓度PDTC作用于骨肉瘤MG-63细胞24 h后,各组细胞的Livin和Caspase-9蛋白的表达水平。[结果]随PDTC浓度增加,骨肉瘤MG-63细胞后其增殖活性呈下降趋势,作用时间越长细胞的增殖活性越低,而细胞的凋亡率呈上升趋势(P<0.05)。随PDTC浓度增加各组细胞中Livin蛋白的表达呈下降趋势,而Caspase9蛋白表达呈上升趋势(P<0.05)。[结论]PDTC可诱导人骨肉瘤MG-63细胞凋亡,其机制可能与PDTC抑制NF-kB传导通路,下调Livin表达继而上调Caspase-9表达有关。[Objective]To detect the effect of pyrrolidine dithiocarbamate（PDTC） on apoptosis and expression of livin and caspase-9 on human osteosarcoma cell MG-63. [Methods]Human osteosarcoma cell MG-63 was cultured in vitro.At 24,48 and 72 hous after treatment of PDTC at different concentration（50,100,200 and 400 μmol/L）,MTT assay was used to observe the growth inhibition of osteosarcoma cell MG-63.The cell apoptosis was assessed by flow cytometry.Protein of livin and caspase-9 was detected by Western blot,at 24 hours after treatment of PDTC at different concentration.[Results]When PDTC was added,growth inhibition and increased apoptosis of MG-63 cells were detected,showing a dose and time dependent manner.PDTC also down-regulated the level of the protein expression of livin（P0.05）,and elevated the protein expression of caspase-3（P0.05）.[Conclusion]PDTC can induce apoptosis of human osteosarcoma cell MG-63,which may be correlated with down-regulation of livin and up-regulation of caspase-9 expression.

关 键 词：吡咯烷二硫代氨基甲酸盐 骨肉瘤 细胞凋亡 LIVIN Caspase-9 

分 类 号：R738.1[医药卫生—肿瘤]