肝细胞癌肝内HLA-DR和CⅡTA的表达  

Hepatic expression of HLA-DR and CⅡTA in hepatocellular carcinoma

作　　者：刘玉元[1] 张卿[1] 肖吉[1] 张绪清[1] 孙振凯[2] 

机构地区：[1]第三军医大学西南医院全军感染病研究所,重庆400038 [2]第三军医大学西南医院肝胆外科,重庆400038

出　　处：《免疫学杂志》2011年第3期199-202,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(30872229)

摘　　要：目的探讨人类白细胞抗原DR(human leucocyte antigen DR,HLA-DR)和Ⅱ类反式激活因子(classⅡtransactivator,CⅡTA)在肝细胞癌(hepatocellular carcinoma,HCC)患者癌组织与癌周肝组织中的表达水平及其意义。方法用逆转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测33例乙型肝炎相关性肝细胞癌患者手术切除的肝癌组织和癌周肝组织中CⅡTA mRNA的表达状况,并用免疫组织化学染色检测肝癌组织和癌周肝组织中HLA-DR和CⅡTA蛋白的表达水平。结果癌周肝组织中HLA-DR蛋白、CⅡTA蛋白与mRNA表达阳性检出率均为100%,肝癌组织中HLA-DR蛋白与CⅡTA蛋白表达阳性检出率分别为24.2%和33.3%,CⅡTA mRNA未检测到阳性表达,两者间差异显著(P<0.05);33例患者的肝癌细胞中均缺乏HLA-DR与CⅡTA蛋白表达,而癌周组织肝细胞中均有不同程度HLA-DR与CⅡTA蛋白表达;组织中HLA-DR表达水平与CⅡTA表达水平呈明显正相关(rs=0.9085,P<0.05)。结论肝癌细胞缺乏HLA-DR表达,与其不表达CⅡTA有关,并可能在肝癌细胞免疫逃逸中起重要作用。In this study,we aimed to investigate the expression of human leucocyte antigen DR（HLA-DR） and classⅡ transactivator（CⅡTA） in the tumor and paired adjacent non-tumor liver tissue of hepatocellular carcinoma（HCC）,as well as their roles in the pathogenesis of HCC.We used reverse transcription-polymerase chain reaction（RT-PCR） to detect the expression of CⅡTA mRNA in tumor and paired adjacent non-tumor liver tissues from 33 patients with hepatitis B related HCC,while immunohistochemistry staining to determine the expressions of HLA-DR and CⅡTA protein.We found that the detection rates of HLA-DR protein,CⅡTA protein and mRNA were all 100% in adjacent non-tumor liver tissues,but only 24.2%,33.3%,and 0 respectively in tumor tissues（P 0.05）.No expression of HLA-DR and CⅡTA protein was observed on tumor cells from 33 patients with HCC,but the expressions of HLA-DR and CⅡTA protein on hepatocytes were observed in all paired adjacent non-tumor liver tissues of 33 patients with HCC.Furthermore,we found the levels of hepatic HLA-DR expression were positively correlated with the levels of hepatic CⅡTA expression（rs = 0.9085,P 0.05）.The results of the present work imply that dysexpression of HLA-DR on HCC cells is associated with the lack of CⅡTA expression,and may play an important role in immune escaping of HCC cells.

关 键 词：肝细胞癌 Ⅱ类反式激活因子 人类白细胞抗原DR 

分 类 号：R735.7[医药卫生—肿瘤]