消痰散结方对人胃癌裸鼠移植瘤血管生成拟态的影响  

Effects of Xiaotan Sanjie Recipe on Vasculogenic Mimicry of Human Gastric Cancer Xenografts in Nude Mice

作　　者：周伟[1] 李勇进[1] 魏品康[1] 

机构地区：[1]第二军医大学长征医院中医科,上海200003

出　　处：《中国中西医结合杂志》2011年第4期532-536,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.90709044)

摘　　要：目的探讨消痰散结方对人胃癌裸鼠移植瘤血管生成拟态(vasculogenic mimicry,VM)的影响及其机制。方法 30只裸鼠右前肢腋部皮下移植人胃癌瘤块建立荷瘤模型。造模后的荷瘤鼠随机分为模型组(10只)、消痰散结组(10只)和多西环素组(10只),分别给予相应药物灌胃治疗4周后,脱颈椎处死荷瘤鼠。剥取肿瘤,称取瘤质量,计算抑瘤率;计数肿瘤中VM的数量;运用免疫组织化学法检测肿瘤中基质金属蛋白酶2(matrix metalloproteinase-2,MMP-2)和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)蛋白的表达。结果消痰散结组和多西环素组裸鼠移植瘤质量明显低于模型组(P<0.01)。消痰散结组和多西环素组VM个数分别为(24.50±3.03)和(14.70±1.34),与模型组(33.10±2.64)比较,差异有统计学意义(P<0.01)。消痰散结组和多西环素组MMP-2和MMP-9蛋白的阳性表达低于模型组,差异均有统计学意义(P<0.01)。结论消痰散结方可抑制裸鼠移植瘤VM的形成,其机制可能与下调MMP-2和MMP-9蛋白表达有关。Objective To study the effect of Xiaotan Sanjie Recipe（XTSJD） and its mechanism on vasculogenic mimicry（VM） of human gastric cancer xenografts in nude mice.Methods The tumor-bearing mice model was established by subcutaneous inoculating with xenografts of human gastric cancer into the right armpit of 30 BALB/c nude mice.After modeling,the tumor-bearing mice were randomly divided into the normal saline group,the XTSJD group,and the doxycycline hyclate（DH） group,10 in each.And the mice were administered with corresponding medicine by gastrogavage for 4 weeks.Then all mice were killed by cervical dislocation.The tumor mass were weighed and the tumor inhibition rate calculated.The amount of VM in tumor was counted.Expressions of matrix metalloproteinase（MMP）-2 and MMP-9 were tested using immunohistochemical method.Results Tumor weight in the XTSJD group and the DH group decreased significantly when compared with the NS group（P0.01）.The amount of VM in the XTSJD group（24.50±3.03） and the DH group（14.70±1.34） was significantly less than that in the NS group（33.10±2.64）（P0.01）.The positive expressions of MMP-2 and MMP-9 in the XTSJD group and the DH group was significantly lower than that in the NS group（P0.01）.Conclusion XTSJD could inhibit the formation of VM in xenografted tumor of nude mice.The mechanism might be correlated with the down-regulation of MMP-2 and MMP-9 expressions.

关 键 词：消痰散结方 胃癌 血管生成拟态 基质金属蛋白酶 

分 类 号：R735.2[医药卫生—肿瘤]