抵抗素体外对胆管癌细胞侵袭和转移的影响及其机制  

Effects of resistin on invasion and metastasis of cholangiocarcinoma cell in vitro

作　　者：吴新权[1] 傅赞[1] 冯湘君[1] 张俊华[1] 孙跃明[1] 

机构地区：[1]南京医科大学第一附属医院微创外科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2011年第3期347-350,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助(BK2006230)

摘　　要：目的:观察重组人抵抗素(resistin)对胆管癌细胞系QBC939增殖、侵袭、转移及基质金属蛋白酶-2(MMP-2)表达的影响。方法:分别采用噻唑蓝(MTT)比色法、Matrigel侵袭实验和迁移实验检测不同浓度的抵抗素对QBC939细胞增殖、侵袭和迁移能力的影响;采用Western blot检测抵抗素对QBC939细胞内MMP-2蛋白表达的影响。结果:当抵抗素浓度高于10 ng/ml时,对QBC939细胞增殖具有明显的促进作用(P<0.01),并呈一定的剂量依赖性;经10、50、100 ng/ml抵抗素处理24 h后,QBC939细胞体外侵袭及迁移能力均较空白对照组明显增强(P<0.01);Western blot法检测抵抗素作用QBC939细胞24 h后MMP-2表达水平显著增加。结论:抵抗素对人胆管癌QBC939细胞的侵袭和转移能力有明显的促进作用,该作用机制可能与提高MMP-2表达水平有关。Objective：To observe the effects of recombinant resistin（RR） on proliferation,invasion,metastasis and matrix metalloprotease-2（MMP-2） expression of human cholangiocarcinoma cell line QBC939.Methods：MTT and Transwell were used to analyze the effects of different concentrations of RR on proliferation,invasion and migration of QBC939 cells;the expression level of MMP-2 protein in QBC939 cells was examined by Western blot.Results：RR significantly promoted the proliferation of QBC939 cells when the concentration of RR was higher than 10 ng/ml（P 〈 0.01）,the effect was dose-dependent to some extent.After treated with 10,50,100 ng/ml RR for 24 h,the capacity of invasion and migration of QBC939 cells were significantly stronger in RR group than in control group（P 〈 0.01）.The expression of MMP-2 protein was up-regulated significantly in QBC939 cells treated with resistin for 24h.Conclusion：RR significantly increases the invasion and metastasis of QBC939 cells in vitro,and the possible mechanism may be related with up-regulation of MMP-2 level.

关 键 词：胆管癌 抵抗素 侵袭 转移 基质金属蛋白酶-2 

分 类 号：R735.8[医药卫生—肿瘤]