TNF-α通过TNF-R1促进小鼠肾上腺皮质细胞系Y1凋亡  

TNF-α induces the apoptosis of Y1 cells through TNF-R1 pathway

作　　者：夏海鸣[1] 方媛[2] 黄培林[1] 

机构地区：[1]东南大学医学院病理学与病理生理学系,江苏南京210009 [2]东南大学附属第二医院生物治疗中心,江苏南京210003

出　　处：《南京医科大学学报（自然科学版）》2011年第3期364-368,373,共6页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市卫生局医学重点科技发展资金资助(ZKX08033);南京市科技发展项目(200901093)

摘　　要：目的:探讨肿瘤坏死因子α(TNF-α)对小鼠肾上腺皮质细胞(Y1细胞)caspase8/3的影响是否主要通过TNF-R1。方法:小RNA干扰法下调Y1细胞中TNF-R1基因的表达,分光光度法分别检测一定浓度TNF-α对Y1细胞及TNF-R1基因干扰抑制后的Y1(miRNA-Y1)细胞caspase8/caspase3影响。结果:miRNA-Y1细胞TNF-R1基因的干扰效率为71.74%;TNF-α处理Y1细胞后,细胞caspase8/3活性明显升高(P<0.05);TNF-α处理miRNA-Y1细胞,细胞caspase8/3活性与未处理组相比无显著差异(P>0.05)。结论:TNF-α对Y1细胞caspase8/3活性的影响主要是通过TNF-R1受体实现的。Objective：To determine whether the effect of TNF-α on caspase8/3 activity in mouse adrenal cortex Y1 cells is mediated by TNF-R1.Methods：The expression of TNF-R1 was down-regulated by short hairpin RNAs（shRNAs） in Y1 cells which expressed TNF-R1 natively.The effect of TNF-α on caspase8/3 activity in native Y1 cells and miRNA-Y1 cells was evaluated by spectrophotometer.Results：Interference efficiency of shRNAs on TNF-R1 expression in miRNA-Y1 cells was 71.25%.The activity of caspase8/3 increased significantly in Y1 cells（P 〈 0.05） and unchanged in miRNA-Y1 cells after treated with TNF-α.Conclusion：The effect of TNF-α on caspase8/3 activity is mainly mediated by TNF-R1.

关 键 词：肿瘤坏死因子α Y1细胞 CASPASE SHRNA TNF-R1 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]